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Literature summary for 1.7.6.1 extracted from

  • Abbruzzetti, S.; Allegri, A.; Bidon-Chanal, A.; Ogata, H.; Soavi, G.; Cerullo, G.; Bruno, S.; Montali, C.; Luque, F.J.; Viappiani, C.
    Electrostatic tuning of the ligand binding mechanism by Glu27 in nitrophorin 7 (2018), Sci. Rep., 8, 10855 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.6.1

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme E27V, vapor diffusion method, using 0.33% (w/v) anthrone, 0.33% (w/v) Congo Red, 0.33% (w/v) N-(2-acetamido)-2-aminoethanesulfonic acid, 0.0 2M HEPES sodium pH 6.8 Rhodnius prolixus

Protein Variants

Protein Variants Comment Organism
E27Q the mutation results in progressive loss of the characteristic pH sensitivity of the ligand binding rate observed for the wild type protein Rhodnius prolixus
E27V the mutation results in progressive loss of the characteristic pH sensitivity of the ligand binding rate observed for the wild type protein Rhodnius prolixus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 nitrite + 2 H+ Rhodnius prolixus
-
 2 nitric oxide + nitrate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodnius prolixus Q6PQK2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
saliva
-
 Rhodnius prolixus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 nitrite + 2 H+
-
 Rhodnius prolixus 2 nitric oxide + nitrate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Nitrophorin 7
-
 Rhodnius prolixus
NP7
-
 Rhodnius prolixus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Rhodnius prolixus