General Stability | Organism |
---|---|
enzyme requires high salt concentration for stability and activity | Haloferax alexandrinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax alexandrinus | M0HXK1 | - |
- |
Haloferax alexandrinus JCM 10717 | M0HXK1 | - |
- |
Purification (Comment) | Organism |
---|---|
using Sepharose 4B, DEAE cellulose, Sephacryl S-300 chromatographies | Haloferax alexandrinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
pH not specified in the publication, temperature not specified in the publication | Haloferax alexandrinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme does not use electrons from either NADH or NADPH. Dithionite is not able to reduce nitrate in the absence of reduced methyl viologen | Haloferax alexandrinus | ? | - |
- |
|
additional information | enzyme does not use electrons from either NADH or NADPH. Dithionite is not able to reduce nitrate in the absence of reduced methyl viologen | Haloferax alexandrinus JCM 10717 | ? | - |
- |
|
reduced methyl viologen + nitrate | - |
Haloferax alexandrinus | methyl viologen + nitrite | - |
? | |
reduced methyl viologen + nitrate | - |
Haloferax alexandrinus JCM 10717 | methyl viologen + nitrite | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Haloferax alexandrinus |
Synonyms | Comment | Organism |
---|---|---|
C452_13229 | - |
Haloferax alexandrinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Haloferax alexandrinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | presence of Fe-S clusters, with peak up to 450 nm in the fully reduced protein | Haloferax alexandrinus |