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Literature summary for 1.8.1.15 extracted from
- Substrate-induced structural alterations of Mycobacterial mycothione reductase and critical residues involved (2018), FEBS Lett., 592, 568-585 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D158A/Y159A | the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme | Mycobacterium tuberculosis |
| S144A | the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme | Mycobacterium tuberculosis |
| S268A | the mutant exhibits enhanced NADPH oxidation rate compared to the wild type enzyme | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mycothione + NADPH + H+ | Mycobacterium tuberculosis | - |
2 mycothiol + NADP+ | - |
? |  |
| mycothione + NADPH + H+ | Mycobacterium tuberculosis H37Rv | - |
2 mycothiol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mycothione + NADPH + H+ | - |
Mycobacterium tuberculosis | 2 mycothiol + NADP+ | - |
? | |
| mycothione + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | 2 mycothiol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 49800, apoenzyme in solution, calculated from amino acid sequence | Mycobacterium tuberculosis |
| homotetramer | 4 * 49800, enzyme bound to NADPH, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MTR | - |
Mycobacterium tuberculosis |
| NADPH-dependent mycothione reductase | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Mycobacterium tuberculosis | |
| NADPH | dependent on | Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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