Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Marichromatium gracile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0132 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile | |
0.097 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.98 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
6.9 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49028 | - |
2 * 49028, calculation from sequence | Marichromatium gracile |
49030 | - |
2 * 49030, nanospray ionization mass spectrometry | Marichromatium gracile |
98000 | - |
gel filtration | Marichromatium gracile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione amide disulfide + NADH + H+ | Marichromatium gracile | - |
2 glutathione amide + NAD+ | - |
? | |
glutathione amide disulfide + NADH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione amide + NAD+ | - |
? | |
glutathione amide disulfide + NADPH + H+ | Marichromatium gracile | - |
2 glutathione amide + NADP+ | - |
? | |
glutathione amide disulfide + NADPH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione amide + NADP+ | - |
? | |
glutathione disulfide + NADH + H+ | Marichromatium gracile | - |
2 glutathione + NAD+ | - |
? | |
glutathione disulfide + NADH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marichromatium gracile | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Marichromatium gracile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione amide disulfide + NADH + H+ | - |
Marichromatium gracile | 2 glutathione amide + NAD+ | - |
? | |
glutathione amide disulfide + NADH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione amide + NAD+ | - |
? | |
glutathione amide disulfide + NADPH + H+ | - |
Marichromatium gracile | 2 glutathione amide + NADP+ | - |
? | |
glutathione amide disulfide + NADPH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione amide + NADP+ | - |
? | |
glutathione disulfide + NADH + H+ | - |
Marichromatium gracile | 2 glutathione + NAD+ | - |
? | |
glutathione disulfide + NADH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49028, calculation from sequence | Marichromatium gracile |
dimer | 2 * 49030, nanospray ionization mass spectrometry | Marichromatium gracile |
Synonyms | Comment | Organism |
---|---|---|
GAR | - |
Marichromatium gracile |
garB | - |
Marichromatium gracile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
146 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile | |
186.8 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile | |
249.7 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | formation of a charge-transfer complex between one of the newly reduced active site thiols and the oxidized FAD | Marichromatium gracile | |
NADH | preferred cofactor | Marichromatium gracile |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
21.2 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile | |
2574 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile | |
14150 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile |