KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NADPH | pH 8.0, 80°C | Pyrococcus furiosus | |
1.25 | - |
(sulfide)n+1 | pH 8.0, 80°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains no metal | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 52000 + 1 * 29000, | Pyrococcus furiosus |
52000 | - |
1 * 52000 + 1 * 29000, | Pyrococcus furiosus |
90000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(sulfide)n+1 + NADPH + H+ | Pyrococcus furiosus | NADH is not utilized as an electron donor for polysulfide reduction. Polysulfide reduction is catalyzed with reduced ferredoxin as an electron donor. However, the rate of sulfide production, at least in vitro, is much lower than when the enzyme uses NADPH to provide reductant for polysulfide reduction | hydrogen sulfide + (sulfide)n + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U195 and Q8U194 | Q8U195: subunit alpha, and Q8U194: subunit beta | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
pH 8.0, 80°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(sulfide)n+1 + NADPH + H+ | NADH is not utilized as an electron donor for polysulfide reduction. Polysulfide reduction is catalyzed with reduced ferredoxin as an electron donor. However, the rate of sulfide production, at least in vitro, is much lower than when the enzyme uses NADPH to provide reductant for polysulfide reduction | Pyrococcus furiosus | hydrogen sulfide + (sulfide)n + NADP+ | - |
? | |
colloidal sulfur + NADPH + H+ | the relative activity is about 40% compared to the activity with polysulfide | Pyrococcus furiosus | ? | - |
? | |
additional information | the enzyme also catalyzes ferredoxin:NADP+ reductase activity | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 52000 + 1 * 29000, | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
SuDH | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
82 | - |
the enzyme shows a 50% increase in activity after 12 h | Pyrococcus furiosus |
95 | - |
half-life: 12 h | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | the enzyme contains approximately four iron-sulfur centers, 11 iron and 6 acid-labile sulfide atoms per mol | Pyrococcus furiosus | |
flavin | enzyme contains flavin | Pyrococcus furiosus | |
NADPH | NADH was not utilized as an electron donor for polysulfide reduction | Pyrococcus furiosus |