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Literature summary for 1.8.1.4 extracted from
- Isolation und Eigenschaften von Liponsuredehydrogenase aus Chloroplasten (1968), Z. Pflanzenphysiol., 58, 193-206.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors | Spinacia oleracea |  |
| EDTA | NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors | Spinacia oleracea | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | 0.3 mM, 50% inhibition | Spinacia oleracea | |
| additional information | no inhibition by iodoacetate | Spinacia oleracea | |
| PCMB | 0.1 mM, 50% inhibition | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0215 | - |
NADH | - |
Spinacia oleracea | |
| 2.1 | - |
lipoic acid | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors | Spinacia oleracea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Spinacia oleracea | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| dihydrolipoamide + NAD+ | - |
Spinacia oleracea | lipoamide + NADH | - |
r | |
| lipoic acid + NADH + H+ | - |
Spinacia oleracea | dihydrolipoic acid + NAD+ | - |
r | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Spinacia oleracea | ? + NAD+ | - |
? | |
| vitamin K5 + NADH | - |
Spinacia oleracea | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors | Spinacia oleracea | |
| NADH | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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