Cloned (Comment) | Organism |
---|---|
expression of mutant T44V in Escherichia coli strain XL1-Blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T44V | site-directed mutagenesis, mutation of Thr44 of the FAD-binding region to Val, corresponding to the prokaryotic sequence, results in 2.2fold reduced activity with a slightly different microenvironment at the FAD-binding site | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50216 | - |
2 * 50216, amino acid sequence calculation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant T44V from Escherichia coli strain XL1-Blue | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
280 | - |
purified recombinant mutant T44V enzyme | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50216, amino acid sequence calculation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
More | enzyme is a member of the pyridine nucleotide-disulfide oxidoreductase family of enzymes, enzyme is the E3 component of three different 2-ketoacid dehydrogenase multienzyme complexes, i.e. the pyruvate, 2-ketoglutarate, and branched chain 2-keto acid dehydrogenase complexes | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD as prosthetic group per enzyme subunit | Homo sapiens |