Application | Comment | Organism |
---|---|---|
degradation | N286 and D320 play a role in the catalytic function of the E3 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
His-tagged wild-type and mutant proteins overexpressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D320N | 48.60% specific activity of the wild type enzyme, 82.7% of FAD content compared to that of the wild-type enzyme | Homo sapiens |
D320N | specific activity is 48.6% to that of the wild-type E3. About 82.7% of FAD content compared to that of wild-type E3. Forms the dimer | Homo sapiens |
N286D | 30.84% specific activity of the wild type enzyme, 96% of FAD content compared to that of the wild-type enzyme | Homo sapiens |
N286D | specific activity is 30.84% to that of the wild-type E3. About 96.0% of FAD content compared to that of wild-type E3. Forms the dimer | Homo sapiens |
N286Q | 24.57% specific activity of the wild type enzyme, 99.4% of FAD content compared to that of the wild-type enzyme | Homo sapiens |
N286Q | specific activity is 24.57% to that of the wild-type E3. About 99.4% of FAD content compared to that of wild-type E3. Forms the dimer | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | wild-type | Homo sapiens | |
0.04 | - |
NADH | wild type enzyme | Homo sapiens | |
0.05 | - |
Lipoamide | mutant enzyme N286Q | Homo sapiens | |
0.05 | - |
Lipoamide | mutant N286Q | Homo sapiens | |
0.07 | - |
Lipoamide | mutant enzyme N286D | Homo sapiens | |
0.07 | - |
Lipoamide | mutant N286D | Homo sapiens | |
0.13 | - |
NADH | mutant enzyme N286D | Homo sapiens | |
0.13 | - |
NADH | mutant N286D | Homo sapiens | |
0.14 | - |
NADH | mutant enzyme N286Q | Homo sapiens | |
0.14 | - |
NADH | mutant N286Q | Homo sapiens | |
0.16 | - |
NAD+ | mutant enzyme N286Q | Homo sapiens | |
0.16 | - |
NAD+ | mutant N286Q | Homo sapiens | |
0.2 | - |
dihydrolipoamide | mutant enzyme N286Q | Homo sapiens | |
0.2 | - |
dihydrolipoamide | mutant N286Q | Homo sapiens | |
0.22 | - |
NAD+ | wild-type | Homo sapiens | |
0.22 | - |
NAD+ | wild type enzyme | Homo sapiens | |
0.24 | - |
NADH | mutant enzyme D320N | Homo sapiens | |
0.24 | - |
NADH | mutant D320N | Homo sapiens | |
0.3 | - |
NAD+ | mutant enzyme N286D | Homo sapiens | |
0.3 | - |
NAD+ | mutant N286D | Homo sapiens | |
0.34 | - |
NAD+ | mutant enzyme D320N | Homo sapiens | |
0.34 | - |
NAD+ | mutant D320N | Homo sapiens | |
0.38 | - |
dihydrolipoamide | wild-type | Homo sapiens | |
0.38 | - |
dihydrolipoamide | wild type enzyme | Homo sapiens | |
0.46 | - |
Lipoamide | mutant enzyme D320N | Homo sapiens | |
0.46 | - |
Lipoamide | mutant D320N | Homo sapiens | |
0.56 | - |
Lipoamide | wild-type | Homo sapiens | |
0.56 | - |
Lipoamide | wild type enzyme | Homo sapiens | |
0.7 | - |
dihydrolipoamide | mutant enzyme N286D | Homo sapiens | |
0.7 | - |
dihydrolipoamide | mutant N286D | Homo sapiens | |
1.21 | - |
dihydrolipoamide | mutant enzyme D320N | Homo sapiens | |
1.21 | - |
dihydrolipoamide | mutant D320N | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
wild-type and mutants, molecular sieving HPLC | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09622 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Homo sapiens |
wild-type and mutant proteins, highly purified | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild type enzyme: 586.50 units/mg protein, mutant N286D: 179.65 units/mg protein, mutant N286Q: 143.15 units/mg protein, mutant D320N: 283.10 units/mg protein | Homo sapiens |
143.1 | - |
mutant N286Q | Homo sapiens |
179.6 | - |
mutant N286D | Homo sapiens |
283.1 | - |
mutant D320N | Homo sapiens |
586.5 | - |
wild-type | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? | |
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
r | |
lipoamide + NADH | - |
Homo sapiens | dihydrolipoamide + NAD+ | - |
r | |
lipoamide + NADH + H+ | - |
Homo sapiens | dihydrolipoamide + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | wild-type and mutants, molecular sieving HPLC | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
Lipoamide | mutant enzyme D320N | Homo sapiens | |
67 | - |
Lipoamide | mutant D320N | Homo sapiens | |
107 | - |
dihydrolipoamide | mutant enzyme D320N | Homo sapiens | |
107 | - |
dihydrolipoamide | mutant D320N | Homo sapiens | |
116 | - |
dihydrolipoamide | mutant enzyme N286Q | Homo sapiens | |
116 | - |
dihydrolipoamide | mutant N286Q | Homo sapiens | |
134 | - |
Lipoamide | mutant enzyme N286D | Homo sapiens | |
134 | - |
Lipoamide | mutant N286D | Homo sapiens | |
148 | - |
Lipoamide | mutant enzyme N286Q | Homo sapiens | |
148 | - |
Lipoamide | mutant N286Q | Homo sapiens | |
159 | - |
dihydrolipoamide | mutant enzyme N286D | Homo sapiens | |
159 | - |
dihydrolipoamide | mutant N286D | Homo sapiens | |
505 | - |
dihydrolipoamide | wild-type | Homo sapiens | |
505 | - |
dihydrolipoamide | wild type enzyme | Homo sapiens | |
519 | - |
Lipoamide | wild-type | Homo sapiens | |
519 | - |
Lipoamide | wild type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |