Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-Bis(2-chloroethyl)-1-nitrosourea | significant inhibition of recombinant isoform GR1 is observed at high concentrations of 1 mM and above | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADPH | in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 | Arabidopsis thaliana | |
0.077 | - |
glutathione disulfide | in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isoform GR2 | Arabidopsis thaliana | 9507 | - |
cytosol | isoform GR1 | Arabidopsis thaliana | 5829 | - |
mitochondrion | isoform GR2 | Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57100 | - |
2 * 57100, calculated for GR1 including the N-terminal His-tag | Arabidopsis thaliana |
60000 | - |
2 * 60000, SDS-PAGE | Arabidopsis thaliana |
120000 | - |
purified native isoform GR1, gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Arabidopsis thaliana | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Arabidopsis thaliana |
homodimer | 2 * 57100, calculated for GR1 including the N-terminal His-tag | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Arabidopsis thaliana |
GR1 | cytosolic isoform, about 65% of the total glutathione reductase activity is attributed to isoform GR1 | Arabidopsis thaliana |
GR2 | isoform dual-targeted to chloroplasts and mitochondria, about 35% of the total glutathione reductase activity is attributed to isoform GR2 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.6 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of isoform GR1 prevents survival due to a pollen lethal phenotype | Arabidopsis thaliana |