Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Drosophila melanogaster |
expressed in Escherichia coli ER2566 cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
0.41 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
0.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
0.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
0.83 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
4.1 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Drosophila melanogaster | |
selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Mus musculus | |
selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Rattus norvegicus | |
selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Caenorhabditis elegans | |
selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Anopheles gambiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
12% reducing SDS-PAGE | Drosophila melanogaster |
55000 | - |
12% reducing SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | Drosophila melanogaster | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | Mus musculus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | Rattus norvegicus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | Caenorhabditis elegans | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | Anopheles gambiae | - |
thioredoxin disulfide + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles gambiae | - |
- |
- |
Caenorhabditis elegans | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Drosophila melanogaster |
DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Caenorhabditis elegans | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Anopheles gambiae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | - |
Drosophila melanogaster | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | - |
Caenorhabditis elegans | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | - |
Anopheles gambiae | thioredoxin disulfide + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Drosophila melanogaster |
thioredoxin reductase | - |
Mus musculus |
thioredoxin reductase | - |
Rattus norvegicus |
thioredoxin reductase | - |
Caenorhabditis elegans |
thioredoxin reductase | - |
Anopheles gambiae |
TR2 | - |
Caenorhabditis elegans |
TR3 | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
2.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
2.62 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
20.85 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
21.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
48.42 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 6.5 | Mus musculus |
7.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.5 | Drosophila melanogaster |
8 | 8.5 | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Drosophila melanogaster | |
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Caenorhabditis elegans | |
NADPH | - |
Anopheles gambiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
4.78 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
5.27 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
5.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
45.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
58.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus |