Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C124A | reduced activity compared to wild type enzyme | Homo sapiens |
C15A | reduced activity compared to wild type enzyme | Homo sapiens |
C15A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
C15A/C74A/C85A/C124A | reduced activity compared to wild type enzyme | Homo sapiens |
C74A/C85A | reduced activity compared to wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
cytochrome c | wild type enzyme | Homo sapiens | |
1.3 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
1.7 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
1.8 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
2 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
2 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
2.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
in 8M urea | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + cytochrome c | - |
Homo sapiens | dithiothreitol disulfide + reduced cytochrome c | - |
? | |
dithiothreitol + O2 | - |
Homo sapiens | dithiothreitol disulfide + H2O2 | - |
? | |
R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | disulfide-bridged dimer (linked via C15-C124) with two free cysteine residues (C74 and 85) per monomer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
flavin-dependent sulfhydryl oxidase | - |
Homo sapiens |
flavin-linked sulfhydryl oxidase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
dithiothreitol | mutant enzyme C74A/C85A | Homo sapiens | |
0.867 | - |
dithiothreitol | mutant enzyme C15A | Homo sapiens | |
0.9 | - |
dithiothreitol | mutant enzyme C124A | Homo sapiens | |
0.95 | - |
dithiothreitol | mutant enzyme C15A/C124A | Homo sapiens | |
1.1 | - |
dithiothreitol | wild type enzyme | Homo sapiens | |
1.25 | - |
dithiothreitol | mutant enzyme C15A/C74A/C85A/C124A | Homo sapiens | |
4.5 | - |
cytochrome c | wild type enzyme | Homo sapiens |