Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutants in Escherichia coli, expression as N- or C-terminally 6His-tagged protein lowers the recombinant expression level to 3% of total enzyme expressed, labeling of expressed wild-type with 75SeMet | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a non-selenomethionine mutant of MsrB by site-directed mutagenesis, exchange of the selenomethionine by Cys, Ala, or Ser, the Cys-enzyme shows reduced activity, the Ser- and Ala-enzymes are inactive, substrate specificity, overview | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
x * 13000, native wild-type MsrB, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | Mus musculus | together with the enzyme MsrA, EC 1.8.4.11, which is absolutely specific for the S-form substrate, the enzyme can repair methionine-damaged proteins and salvage free methionine under oxidative stress int the living cell | L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
selenomethionine-containing enzyme MsrB | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged wild-type and mutant MsrB to homogeneity | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | selenomethionine is essential for MsrB activity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | together with the enzyme MsrA, EC 1.8.4.11, which is absolutely specific for the S-form substrate, the enzyme can repair methionine-damaged proteins and salvage free methionine under oxidative stress int the living cell | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | substrate specificity | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13000, native wild-type MsrB, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase B | - |
Mus musculus |
MsrB | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mus musculus |