Cloned (Comment) | Organism |
---|---|
expresion in Escherichia coli BL-21 Star (DE3) | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, crystal structure of HpMsrAB C44S/C318S at 2.2 A, which shows that a linker region (Hpiloop, 193-205) between two domains interacts with each HpMsrA or HpMsrB domain via three salt bridges (E193-K107, D197-R103, and K200-D339) | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
D197A | kcat/Km for dabsyl-L-methionine (R)-sulfoxide is 2.6fold lower than wild-type value | Helicobacter pylori |
E193A | kcat/Km for dabsyl-L-methionine (R)-sulfoxide is 2.4fold lower than wild-type value | Helicobacter pylori |
E193A/D197A | kcat/Km for dabsyl-L-methionine (R)-sulfoxide is 3.1fold lower than wild-type value | Helicobacter pylori |
E339A | kcat/Km for dabsyl-L-methionine (R)-sulfoxide is 1.39fold lower than wild-type value | Helicobacter pylori |
Y343F | kcat/Km for dabsyl-L-methionine (R)-sulfoxide is 1.1fold lower than wild-type value | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E339A | Helicobacter pylori | |
0.03 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme Y343F | Helicobacter pylori | |
0.05 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, wild-type enzyme | Helicobacter pylori | |
0.05 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A/D197A | Helicobacter pylori | |
0.06 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme D197A | Helicobacter pylori | |
0.06 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | O25011 | - |
- |
Helicobacter pylori ATCC 700392 | O25011 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol | - |
Helicobacter pylori | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol | - |
Helicobacter pylori ATCC 700392 | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
msrAB | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E339A | Helicobacter pylori | |
0.05 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A/D197A | Helicobacter pylori | |
0.073 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme D197A | Helicobacter pylori | |
0.078 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A | Helicobacter pylori | |
0.087 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme Y343F | Helicobacter pylori | |
0.157 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, wild-type enzyme | Helicobacter pylori |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A/D197A | Helicobacter pylori | |
1.22 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme D197A | Helicobacter pylori | |
1.3 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E193A | Helicobacter pylori | |
2.25 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme E339A | Helicobacter pylori | |
2.88 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, mutant enzyme Y343F | Helicobacter pylori | |
3.1 | - |
dabsyl-L-methionine (R)-sulfoxide | pH 7.5, 37°C, wild-type enzyme | Helicobacter pylori |