Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus ferrooxidans |
Protein Variants | Comment | Organism |
---|---|---|
C128A | the mutant shows strongly reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C128S | the mutant shows strongly reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C160A | the mutant shows severely reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C160S | the mutant shows strongly reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
C356A | inactive | Acidithiobacillus ferrooxidans |
C356S | the mutant shows severely reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
H132A | the mutant shows strongly reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
H198A | the mutant shows severely reduced activity compared to the wild type enzyme | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00297 | - |
Sulfide | wild type enzyme, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.00343 | - |
decylubiquinone | wild type enzyme, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.00425 | - |
decylubiquinone | mutant enzyme C128A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.005 | - |
Sulfide | mutant enzyme H132A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.0054 | - |
decylubiquinone | mutant enzyme H132A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.00553 | - |
Sulfide | mutant enzyme C128S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.0056 | - |
Sulfide | mutant enzyme C128A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.00585 | - |
decylubiquinone | mutant enzyme C128S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | B7JBP8 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfide + decylubiquinone | - |
Acidithiobacillus ferrooxidans | sulfur + decylubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
SQR | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Acidithiobacillus ferrooxidans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
45 | the recombinant enzyme is highly stable when incubated at 0°C, 4°C, 23°C or 30°C with more than 90% of the activity remaining after 7 h. It is less stable at 37°C and loses about 40% of the activity in the first 7.5 h. The activity of the enzyme decreases more rapidly at 45°C with a half-life of approximately 5 h | Acidithiobacillus ferrooxidans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Sulfide | mutant enzyme C160A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.1 | - |
Sulfide | mutant enzyme C356S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.3 | - |
Sulfide | mutant enzyme C198A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
0.5 | - |
Sulfide | mutant enzyme C160S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
1 | - |
Sulfide | mutant enzyme C128A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
1.4 | - |
Sulfide | mutant enzyme H132A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
1.6 | - |
Sulfide | mutant enzyme C128S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
6.5 | - |
Sulfide | wild type enzyme, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Acidithiobacillus ferrooxidans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
180 | - |
Sulfide | mutant enzyme C128A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
240 | - |
decylubiquinone | mutant enzyme C128A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
250 | - |
decylubiquinone | mutant enzyme H132A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
270 | - |
decylubiquinone | mutant enzyme C128S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
270 | - |
Sulfide | mutant enzyme H132A, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
290 | - |
Sulfide | mutant enzyme C128S, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
1890 | - |
decylubiquinone | wild type enzyme, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans | |
2190 | - |
Sulfide | wild type enzyme, at pH 7.0 and 23°C | Acidithiobacillus ferrooxidans |