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Literature summary for 1.8.5.7 extracted from

  • Green, A.R.; Hayes, R.P.; Xun, L.; Kang, C.
    Structural understanding of the glutathione-dependent reduction mechanism of glutathionyl-hydroquinone reductases (2012), J. Biol. Chem., 287, 35838-35848.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.7

Crystallization (Commentary)

Crystallization (Comment) Organism
binary complex structure with glutathionyl-menadione. The structure reveals a large H-site that could accommodate various substituted hydroquinones and a hydrogen network of three Tyr residues that could provide the proton for reductive deglutathionylation Escherichia coli

Protein Variants

Protein Variants Comment Organism
C63A catalytic residue, mutant displays negligible activity Escherichia coli
Y195F catalytic residue, mutant displays negligible activity Escherichia coli
Y253F catalytic residue, mutant displays negligible activity Escherichia coli
Y296F catalytic residue, mutant displays negligible activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 2-(glutathione-S-yl)-hydroquinone wild-type, pH 7.5, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P42620
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 mutant Y253F, pH 7.5, temperature not specified in the publication Escherichia coli
0.19
-
 mutant Y195F, pH 7.5, temperature not specified in the publication Escherichia coli
0.4
-
 mutant Y296F, pH 7.5, temperature not specified in the publication Escherichia coli
8.65
-
 wild-type, pH 7.5, temperature not specified in the publication Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Escherichia coli glutathione disulfide + hydroquinone
-
 ?

Synonyms

Synonyms Comment Organism
glutathionyl-hydroquinone reductase
-
 Escherichia coli
yqjG
-
 Escherichia coli