Literature summary for 1.8.5.B1 extracted from

Loschi, L.; Brokx, S.J.; Hills, T.L.; Zhang, G.; Bertero, M.G.; Lovering, A.L.; Weiner, J.H.; Strynadka, N.C.
Structural and biochemical identification of a novel bacterial oxidoreductase (2004), J. Biol. Chem., 279, 50391-50400 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of catalytic subunit YedY in both the molybdenum- and tungsten-substituted forms, five monomers per asymmetric unit	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
12	-	dimethyl sulfoxide	25°C, pH not specified in the publication	Escherichia coli
22	-	Trimethylamine N-oxide	25°C, pH not specified in the publication	Escherichia coli
27.9	-	tetrahydrothiophene oxide	25°C, pH not specified in the publication	Escherichia coli
46.4	-	Trimethylamine N-oxide	presence of 1 M urea, 25°C, pH not specified in the publication	Escherichia coli
119	-	L-methionine sulfoxide	25°C, pH not specified in the publication	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Molybdenum	the molybdenum ion is coordinated by three sulfur ligands, two contributed from the dithiolene sulfurs of the molybdopterin with a Mo-S distance of 2.4 A. The third sulfur ligand is Sgamma of residue C102 at a distance of 2.4 A	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P76342 and P76343	P76342 i.e. catalytic subunit YedY/MsrP, P76343 i.e. heme-binding subunit YedZ/MsrQ	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dimethyl sulfoxide + reduced benzyl viologen	-	Escherichia coli	dimethyl sulfide + oxidized benzyl viologen + H2O	-	?
L-methionine sulfoxide + reduced benzyl viologen	-	Escherichia coli	L-methionine + oxidized benzyl viologen + H2O	-	?
tetrahydrothiophene oxide + reduced benzyl viologen	-	Escherichia coli	tetrahydrothiophene + oxidized benzyl viologen + H2O	-	?
trimethylamine N-oxide + reduced benzyl viologen	-	Escherichia coli	trimethylamine + oxidized benzyl viologen + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	monomer in solution, light scattering analysis	Escherichia coli

Synonyms

Synonyms	Comment	Organism
YedY	-	Escherichia coli
YedZ	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.83	-	dimethyl sulfoxide	25°C, pH not specified in the publication	Escherichia coli
14.8	-	Trimethylamine N-oxide	presence of 1 M urea, 25°C, pH not specified in the publication	Escherichia coli
18.1	-	tetrahydrothiophene oxide	25°C, pH not specified in the publication	Escherichia coli
19	-	Trimethylamine N-oxide	25°C, pH not specified in the publication	Escherichia coli
25.6	-	L-methionine sulfoxide	25°C, pH not specified in the publication	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
molybdopterin	presence of one molybdenum molybdopterin not conjugated by an additional nucleotide.	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.215	-	L-methionine sulfoxide	25°C, pH not specified in the publication	Escherichia coli
0.319	-	Trimethylamine N-oxide	presence of 1 M urea, 25°C, pH not specified in the publication	Escherichia coli
0.403	-	dimethyl sulfoxide	25°C, pH not specified in the publication	Escherichia coli
0.649	-	tetrahydrothiophene oxide	25°C, pH not specified in the publication	Escherichia coli
0.864	-	Trimethylamine N-oxide	25°C, pH not specified in the publication	Escherichia coli

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Release 2023.1 (January 2023)