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Literature summary for 1.8.5.B1 extracted from

  • Loschi, L.; Brokx, S.J.; Hills, T.L.; Zhang, G.; Bertero, M.G.; Lovering, A.L.; Weiner, J.H.; Strynadka, N.C.
    Structural and biochemical identification of a novel bacterial oxidoreductase (2004), J. Biol. Chem., 279, 50391-50400 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of catalytic subunit YedY in both the molybdenum- and tungsten-substituted forms, five monomers per asymmetric unit Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
dimethyl sulfoxide 25┬░C, pH not specified in the publication Escherichia coli
22
-
Trimethylamine N-oxide 25┬░C, pH not specified in the publication Escherichia coli
27.9
-
tetrahydrothiophene oxide 25┬░C, pH not specified in the publication Escherichia coli
46.4
-
Trimethylamine N-oxide presence of 1 M urea, 25┬░C, pH not specified in the publication Escherichia coli
119
-
L-methionine sulfoxide 25┬░C, pH not specified in the publication Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum the molybdenum ion is coordinated by three sulfur ligands, two contributed from the dithiolene sulfurs of the molybdopterin with a Mo-S distance of 2.4 A. The third sulfur ligand is Sgamma of residue C102 at a distance of 2.4 A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76342 and P76343 P76342 i.e. catalytic subunit YedY/MsrP, P76343 i.e. heme-binding subunit YedZ/MsrQ
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethyl sulfoxide + reduced benzyl viologen
-
Escherichia coli dimethyl sulfide + oxidized benzyl viologen + H2O
-
?
L-methionine sulfoxide + reduced benzyl viologen
-
Escherichia coli L-methionine + oxidized benzyl viologen + H2O
-
?
tetrahydrothiophene oxide + reduced benzyl viologen
-
Escherichia coli tetrahydrothiophene + oxidized benzyl viologen + H2O
-
?
trimethylamine N-oxide + reduced benzyl viologen
-
Escherichia coli trimethylamine + oxidized benzyl viologen + H2O
-
?

Subunits

Subunits Comment Organism
monomer monomer in solution, light scattering analysis Escherichia coli

Synonyms

Synonyms Comment Organism
YedY
-
Escherichia coli
YedZ
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.83
-
dimethyl sulfoxide 25┬░C, pH not specified in the publication Escherichia coli
14.8
-
Trimethylamine N-oxide presence of 1 M urea, 25┬░C, pH not specified in the publication Escherichia coli
18.1
-
tetrahydrothiophene oxide 25┬░C, pH not specified in the publication Escherichia coli
19
-
Trimethylamine N-oxide 25┬░C, pH not specified in the publication Escherichia coli
25.6
-
L-methionine sulfoxide 25┬░C, pH not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
molybdopterin presence of one molybdenum molybdopterin not conjugated by an additional nucleotide. Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.215
-
L-methionine sulfoxide 25┬░C, pH not specified in the publication Escherichia coli
0.319
-
Trimethylamine N-oxide presence of 1 M urea, 25┬░C, pH not specified in the publication Escherichia coli
0.403
-
dimethyl sulfoxide 25┬░C, pH not specified in the publication Escherichia coli
0.649
-
tetrahydrothiophene oxide 25┬░C, pH not specified in the publication Escherichia coli
0.864
-
Trimethylamine N-oxide 25┬░C, pH not specified in the publication Escherichia coli