Cloned (Comment) | Organism |
---|---|
expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3) | Coffea canephora |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution | Coffea canephora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43400 | - |
2 * 43400, about, sequence calculation | Coffea canephora |
80000 | - |
about, recombinant enzyme, gel filtration | Coffea canephora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Coffea canephora | the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 | ? | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea canephora | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea canephora | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea canephora | A4GE70 | DXMT1; var. robusta, bifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration | Coffea canephora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme also catalyzes the reaction of the caffeine synthase, EC 2.1.1.160 | Coffea canephora | ? | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea canephora | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea canephora | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43400, about, sequence calculation | Coffea canephora |
Synonyms | Comment | Organism |
---|---|---|
DXMT1 | - |
Coffea canephora |