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Literature summary for 2.1.1.274 extracted from
- S-Adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases (1999), Arch. Biochem. Biophys., 367, 9-16.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SAMT cDNA expressed with pET-11a/pET-28 vector in Escherichia coli which synthesizes a functional non-tagged/His-tagged SAMT protein | Clarkia breweri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, mild inhibitory effect, less than 35% reduced activity | Clarkia breweri |  |
| Cu2+ | 5 mM, strong inhibitory effect with 50100% inhibition | Clarkia breweri | |
| Fe2+ | 5 mM, strong inhibitory effect with 50100% inhibition | Clarkia breweri | |
| Fe3+ | 5 mM, strong inhibitory effect with 50100% inhibition | Clarkia breweri | |
| Mg2+ | 5 mM, mild inhibitory effect, less than 35% reduced activity | Clarkia breweri | |
| Mn2+ | 5 mM, mild inhibitory effect, less than 35% reduced activity | Clarkia breweri | |
| additional information | 5 mM Na+ and NH4+ have no effect on activity | Clarkia breweri | |
| Zn2+ | 5 mM, strong inhibitory effect with 50100% inhibition | Clarkia breweri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
salicylate | app. Km-value of His-tagged protein, pH 7.5, 20°C | Clarkia breweri | |
| 0.009 | - |
S-adenosyl-L-methionine | app. Km-value of nontagged and His-tagged protein, pH 7.5, 20°C | Clarkia breweri | |
| 0.024 | - |
salicylate | app. Km-value of nontagged protein, pH 7.5, 20°C | Clarkia breweri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 5 mM, stimulates SAMT activity by a factor of 2 | Clarkia breweri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
monomer after Mono-Q chromatography | Clarkia breweri |
| 40300 | - |
calculated from sequence | Clarkia breweri |
| 74500 | - |
native protein | Clarkia breweri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + salicylate | Clarkia breweri | - |
S-adenosyl-L-homocysteine + methyl salicylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | Q9SPV4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography | Clarkia breweri |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + salicylate = S-adenosyl-L-homocysteine + methyl salicylate | the enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase | Clarkia breweri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| petal | highest SAMT activity compared to the levels of activity in all other floral parts or the vegetative tissue | Clarkia breweri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000228 | - |
crude extract, pH 7.5, 20°C | Clarkia breweri |
| 0.00069 | - |
DE-53 DEAE chromatography, pH 7.5, 20°C | Clarkia breweri |
| 0.00087 | - |
HAP chromatography, pH 7.5, 20°C | Clarkia breweri |
| 0.02118 | - |
Mono-Q chromatography, pH 7.5, 20°C | Clarkia breweri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme is also active with benzoic acid resulting in methyl benzoate formation | Clarkia breweri | ? | - |
? | |
| additional information | no activity with 4-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid and (+/-)jasmonic acid | Clarkia breweri | ? | - |
? | |
| S-adenosyl-L-methionine + 3-hydroxybenzoic acid | less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% | Clarkia breweri | S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate | - |
? | |
| S-adenosyl-L-methionine + cinnamic acid | less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% | Clarkia breweri | S-adenosyl-L-homocysteine + methyl cinnamate | - |
? | |
| S-adenosyl-L-methionine + salicylate | - |
Clarkia breweri | S-adenosyl-L-homocysteine + methyl salicylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40300, calculated from sequence | Clarkia breweri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | - |
Clarkia breweri |
| SAMT | - |
Clarkia breweri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | SAMT is 100% stable for 30 min at 20°C and 80% stable for 30 min at 30°C, but after 30 min incubation at 40°C it completely loses activity | Clarkia breweri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
salicylate | nontagged protein, pH 7.5, 20°C | Clarkia breweri | |
| 14 | - |
salicylate | His-tagged protein, pH 7.5, 20°C | Clarkia breweri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Clarkia breweri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | at pH 6.0 activity is 80% of optimal activity, at pH 8.0 it is 75%, and at pH 9 it is less than 50% | Clarkia breweri |
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Release 2023.1 (January 2023)
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