Cloned (Comment) | Organism |
---|---|
Escherichia coli transformed with a high amplifying expression plasmid containing the Escherichia coli thymidylate synthase gene | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystals of TS apoprotein grown using hanging drop method of vapor diffusion, cubic Laue group m3 with a = 133 A | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-fluorouracil | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, crystallographic studies | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Lacticaseibacillus casei |
dimer | 2 * 28000, crystallographic studies | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(+)-methylenetetrahydrofolate | - |
Escherichia coli | |
(+)-methylenetetrahydrofolate | - |
Lacticaseibacillus casei | |
methenyltetrahydropteroylglutamate | - |
Escherichia coli |