Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | - |
Oryctolagus cuniculus |
Cloned (Comment) | Organism |
---|---|
mutations introduced into the cDNA for human cytosolic SHMT and expressed from an Escherichia coli expression system | Homo sapiens |
mutations introduced into the cDNA for rabbit cytosolic SHMT and expressed from an Escherichia coli expression system | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
L474F | shows normal values for kcat and Km for serine, shows lowered affinity (increased dissociation constant) for only the pentaglutamate form of the folate ligand, decreased rates of pyridoxal phosphate addition to the mutant apo enzymes to form the active holo enzymes, thermal stability of SHMT or the rate at which it converts 5,10-methenyl tetrahydropteroyl pentaglutamate to 5-formyl tetrahydropteroyl pentaglutamate not affected | Homo sapiens |
L474F | shows normal values for kcat and Km for serine, shows lowered affinity (increased dissociation constant) for only the pentaglutamate form of the folate ligand, shows decreased rates of pyridoxal phosphate addition to the mutant apo enzymes to form the active holo enzymes, thermal stability of SHMT or the rate at which it converts 5,10-methenyl tetrahydropteroyl pentaglutamate to 5-formyl tetrahydropteroyl pentaglutamate not affected | Oryctolagus cuniculus |
S394N | shows normal values for kcat and Km for serine, has increased dissociation constant values for both glycine and tetrahydrofolate and its pentaglutamate form compared to wild-type enzyme, decreased rates of pyridoxal phosphate addition to the mutant apo enzymes to form the active holo enzymes, thermal stability of SHMT or the rate at which it converts 5,10-methenyl tetrahydropteroyl pentaglutamate to 5-formyl tetrahydropteroyl pentaglutamate not affected | Homo sapiens |
S394N | shows normal values for kcat and Km for serine, has increased dissociation constant values for both glycine and tetrahydrofolate and its pentaglutamate form compared to wild-type enzyme, shows decreased rates of pyridoxal phosphate addition to the mutant apo enzymes to form the active holo enzymes, thermal stability of SHMT or the rate at which it converts 5,10-methenyl tetrahydropteroyl pentaglutamate to 5-formyl tetrahydropteroyl pentaglutamate not affected | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
tetrahydrofolate | mutant L474F | Homo sapiens | |
0.0041 | - |
tetrahydrofolate | wild-type | Homo sapiens | |
0.009 | - |
tetrahydrofolate | mutant L474F | Oryctolagus cuniculus | |
0.01 | - |
tetrahydrofolate | wild-type | Oryctolagus cuniculus | |
0.01 | - |
tetrahydrofolate | mutant S394N | Homo sapiens | |
0.025 | - |
tetrahydrofolate | mutant S394N | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | P07511 | - |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and gel filtration, more than 95% pure | Homo sapiens |
by ammonium sulfate precipitation and gel filtration, more than 95% pure | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyl-tetrahydropteroyl pentaglutamate + glycine + H2O | - |
Homo sapiens | 5-formyl-tetrahydropteroyl pentaglutamate + L-serine | - |
? | |
5,10-methenyl-tetrahydropteroyl pentaglutamate + glycine + H2O | - |
Oryctolagus cuniculus | 5-formyl-tetrahydropteroyl pentaglutamate + L-serine | - |
? | |
L-serine + tetrahydrofolate | - |
Homo sapiens | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | - |
Oryctolagus cuniculus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
serine hydroxymethyltransferase | - |
Oryctolagus cuniculus |
SHMT | - |
Homo sapiens |
SHMT | - |
Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
tetrahydrofolate | mutant S394N, in the presence of pyridoxal phosphate | Homo sapiens | |
0.02167 | - |
tetrahydrofolate | mutant L474F, in the presence of pyridoxal phosphate | Homo sapiens | |
0.033 | - |
tetrahydrofolate | mutant L474F, in the presence of pyridoxal phosphate | Oryctolagus cuniculus | |
0.04 | - |
tetrahydrofolate | wild-type, in the presence of pyridoxal phosphate | Homo sapiens | |
0.045 | - |
tetrahydrofolate | mutant S394N, in the presence of pyridoxal phosphate | Oryctolagus cuniculus | |
0.07 | - |
tetrahydrofolate | wild-type, in the presence of pyridoxal phosphate | Oryctolagus cuniculus |