Application | Comment | Organism |
---|---|---|
analysis | protein sequence of the two isoforms, the short form SHMT-S and the long form SHMT-L, show 59% identity | Leishmania major |
Cloned (Comment) | Organism |
---|---|
both isoforms cloned in frame with EGFP at their C-terminus and transfected into Leishmania major | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-cycloserine | overexpression of SHMT-S increases resistance in a rich folate containing medium | Leishmania major | |
Thiosemicarbazide | poorly active against promastigotes, but SHMT-S transfectants can provide a small but significant resistance | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | SHMT-S | Leishmania major | 5829 | - |
additional information | SHMT-L in an organelle that has hallmarks of the parasite mitochondrion | Leishmania major | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50600 | - |
sequence analysis, SHMT-S | Leishmania major |
53100 | - |
sequence analysis, SHMT-L | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | - |
- |
- |
Leishmania major | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | preferentially expressed | Leishmania infantum | - |
promastigote | - |
Leishmania infantum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | adding 2fold more glycine in the medium increases significantly the expression of SHMT-S and to an even higher level, the expression of SHMT-L, adding 2fold more serine has the reverse effect on the expression of SHMT-L, while the expression of SHMT-S does not change significantly | Leishmania infantum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Leishmania major |
serine hydroxymethyltransferase | - |
Leishmania infantum |
SHMT-L | - |
Leishmania major |
SHMT-L | - |
Leishmania infantum |
SHMT-S | - |
Leishmania major |
SHMT-S | - |
Leishmania infantum |