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Literature summary for 2.3.1.158 extracted from

  • Ghosal, A.; Banas, A.; Stahl, U.; Dahlqvist, A.; Lindqvist, Y.; Stymne, S.
    Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities (2007), Biochim. Biophys. Acta, 1771, 1457-1463.
    View publication on PubMed
Search for more literature for 2.3.1.158

Application

Application Comment Organism
molecular biology the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region. Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane soluble form is produced for comparison with the native membrane bound enzyme Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 SDS-PAGE, after deglycosylation Saccharomyces cerevisiae
80000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein extensive deglycosylation severely decreases the activity Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
the recombinant protein by Ni2+ affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00138
-
 sn-1,2-distearoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
0.001399
-
 sn-1,2-dipalmitoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
0.002453
-
 sn-1,2-dilinolenoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
0.002468
-
 sn-1,2-dioleoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae
0.002597
-
 sn-1,2-dilinoleoylphosphatidylethanolamine as substrate using the membrane bound enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol
-
 Saccharomyces cerevisiae 1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol
-
 ?
sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol
-
 ?
sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylphosphatidylcholine
-
 ?
sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol
-
 Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine
-
 ?
sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol
-
 Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine
-
 ?
sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + 1-oleoylphosphatidylethanolamine
-
 ?
sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol
-
 Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
PDAT
-
 Saccharomyces cerevisiae
phospholipid:diacylglycerol acyltransferase
-
 Saccharomyces cerevisiae
ScPDAT
-
 Saccharomyces cerevisiae