General Stability | Organism |
---|---|
glycerol or 2-mercaptoethanol stabilizes | Sus scrofa |
inactivation at Tris-HCl concentration | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Sus scrofa | |
long-chain 3-oxoacyl-CoA compounds | - |
Sus scrofa | |
N-Methylmaleimide | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
3-oxodecanoyl-CoA | - |
Sus scrofa | |
0.0024 | - |
3-oxooctanoyl-CoA | - |
Sus scrofa | |
0.0083 | - |
3-oxohexanoyl-CoA | - |
Sus scrofa | |
0.0087 | - |
CoA-SH | with acetoacetyl-CoA | Sus scrofa | |
0.017 | - |
acetoacetyl-CoA | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
4 * 46000, SDS-PAGE | Sus scrofa |
200000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + 3-oxoacyl-CoA | Sus scrofa | enzyme catalyses the last step in the beta-oxidation cycle | acyl-CoA + acetyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Sus scrofa |
597 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
25°C, 0.75 M Tris-HCl, pH 8.1, 25% v/v glycerol, 10 mM 2-mercaptoethanol, stable for days | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + 3-oxoacyl-CoA | - |
Sus scrofa | acyl-CoA + acetyl-CoA | - |
r | |
CoA + 3-oxoacyl-CoA | enzyme catalyses the last step in the beta-oxidation cycle | Sus scrofa | acyl-CoA + acetyl-CoA | - |
r | |
CoA + 3-oxodecanoyl-CoA | - |
Sus scrofa | acetyl-CoA + octanoyl-CoA | - |
r | |
CoA + 3-oxohexanoyl-CoA | - |
Sus scrofa | acetyl-CoA + butanoyl-CoA | - |
r | |
CoA + 3-oxooctanoyl-CoA | - |
Sus scrofa | acetyl-CoA + hexanoyl-CoA | - |
r | |
CoA + acetoacetyl-CoA | - |
Sus scrofa | acetyl-CoA + acetyl-CoA | - |
r | |
additional information | acetoacetyl-CoA is a poor substrate | Sus scrofa | ? | - |
? | |
additional information | acts on 3-oxoacyl-CoA compounds of various chain lengths | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, SDS-PAGE | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 8.1, 25% glycerol v/v, stable for days | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.3 | pH 7.6: maximum activity, pH 8.3: about 75% of maximum activity | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
25°C, 25% glycerol v/v, stable for days | Sus scrofa |