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Literature summary for 2.3.1.225 extracted from

  • Dunphy, J. T.; Greentree, W. K.; Manahan, C. L.; Linder, M. E.
    G-protein palmitoyltransferase activity is enriched in plasma membranes (1996), J. Biol. Chem., 271, 7154-7159.
    View publication on PubMed
Search for more literature for 2.3.1.225

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-
additional information low or undetectable levels in Golgi, endoplasmic reticulum, and mitochondria of rat liver, subcellular localization study, overview Rattus norvegicus
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additional information no activity in cytosol, subcellular localization study, overview Bos taurus
-
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plasma membrane high enzyme level Rattus norvegicus 5886
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [Gialpha1]-L-cysteine Bos taurus
-
 [Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine Rattus norvegicus
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine Bos taurus
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 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from brain membranes partially by gel filtration and anion exchange chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
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liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information G-protein substrate specificity of PAT activity, overview. Mutation of the prenylated cysteine residue to serine, C68S, in the gamma2 subunit yields a nonprenylated gamma that heterodimerizes with the beta1 subunit. The mutant betagammaC68S binds to myristoylated rGialpha1, forming a heterotrimer that is acylated in vitro with efficiency similar to that of the wild-type heterotrimer Rattus norvegicus ?
-
 ?
additional information PAT fatty acyl-CoA chain length specificity in vitro, overview. PAT shows G-protein substrate specificity of PAT activity, overview. Mutation of the prenylated cysteine residue to serine, C68S, in the gamma2 subunit yields a nonprenylated gamma that heterodimerizes with the beta1 subunit. The mutant betagammaC68S binds to myristoylated rGialpha1, forming a heterotrimer that is acylated in vitro with efficiency similar to that of the wild-type heterotrimer Bos taurus ?
-
 ?
palmitoyl-CoA + [Gialpha1]-L-cysteine
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 Bos taurus [Gialpha1]-S-palmitoyl-L-cysteine + CoA
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 r
palmitoyl-CoA + [Gialpha1]-L-cysteine G protein alpha subunit, wild-type Gia1 and Gia1G203A mutant form heterotrimers with G protein betagammaC68S, myristoylated or not myristoylated Rattus norvegicus [Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [Gialpha1]-L-cysteine recombinant myristoylated G protein alpha subunit and bovine brain betagamma subunits. G-protein substrate specificity of PAT activity, overview. wild-type Gia1 and Gia1G203A mutant form heterotrimers with G protein betagammaC68S Bos taurus [Gialpha1]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [GOalpha1]-L-cysteine
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 Bos taurus [GOalpha1]-S-palmitoyl-L-cysteine + CoA
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 r
palmitoyl-CoA + [GSalpha1]-L-cysteine Gsa is not a myristoylated protein, but is palmitoylated at Cys3 Bos taurus [GSalpha1]-S-palmitoyl-L-cysteine + CoA
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 r
palmitoyl-CoA + [protein]-L-cysteine
-
 Rattus norvegicus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine
-
 Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Synonyms

Synonyms Comment Organism
Pat
-
 Rattus norvegicus
Pat
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rattus norvegicus
8
-
 assay at Bos taurus

General Information

General Information Comment Organism
additional information role of G-protein betagamma subunits in substrate affinity for PAT may be to provide a mechanism for substrate presentation to PAT. Bos taurus
physiological function the enzyme has a role for regulated cycles of acylation and deacylation accompanying activation of G-protein signal transduction pathways Rattus norvegicus
physiological function the enzyme has a role for regulated cycles of acylation and deacylation accompanying activation of G-protein signal transduction pathways Bos taurus