Protein Variants | Comment | Organism |
---|---|---|
C164R | substitution of the conserved cysteine in the DHHC motif. Overexpression of this mutant allows to partially complement an isoform Swf1 gene deletion and to acylate the Swf1 substrates Tlg1, Syn8, and Snc1. Mutant protein is not acylated | Saccharomyces cerevisiae |
H162Q | substitution in the DHHC motif, mutant is partially active | Saccharomyces cerevisiae |
H162Q/C164R | substitutions in the DHHC motif, mutant is inactive | Saccharomyces cerevisiae |
additional information | substitution of the conserved cysteine in the DHHC motif to DHHA or DHHR still allows for acylation of substrate Chs3. Mutant protein is not acylated | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04629 | - |
- |
Saccharomyces cerevisiae | Q12006 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
palmitoylation | enzyme undergoes autoacylation | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [Chs3]-L-cysteine | - |
Saccharomyces cerevisiae | [Chs3]-S-palmitoyl-L-cysteine + CoA | - |
? | |
palmitoyl-CoA + [Pfa4]-L-cysteine | autoacylation | Saccharomyces cerevisiae | [Pfa4]-S-palmitoyl-L-cysteine + CoA | - |
? | |
palmitoyl-CoA + [Snc1]-L-cysteine | - |
Saccharomyces cerevisiae | [Snc1]-S-palmitoyl-L-cysteine + CoA | - |
? | |
palmitoyl-CoA + [Swf1]-L-cysteine | autoacylation | Saccharomyces cerevisiae | [Swf1]-S-palmitoyl-L-cysteine + CoA | - |
? | |
palmitoyl-CoA + [Syn8]-L-cysteine | - |
Saccharomyces cerevisiae | [Syn8]-S-palmitoyl-L-cysteine + CoA | - |
? | |
palmitoyl-CoA + [Tlg1]-L-cysteine | - |
Saccharomyces cerevisiae | [Tlg1]-S-palmitoyl-L-cysteine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pfa4 | - |
Saccharomyces cerevisiae |
Swf1 | - |
Saccharomyces cerevisiae |