Protein Variants | Comment | Organism |
---|---|---|
additional information | T-DNA insertion mutants lead to complete disruption of the respective enzyme genes, mutants for cytosolic (csat) and plastidic (psat) enzyme isoforms, and double mutants (dmsat) of the 2 isoforms by crossing | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | feedback inhibition; feedback inhibition | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | SERAT1.1 | Arabidopsis thaliana | 5829 | - |
mitochondrion | compartment with largest serine acetyltransferase activity of total serine acetyltransferase activity independent of cytosolic serine acetyltransferase activity | Arabidopsis thaliana | 5739 | - |
mitochondrion | compartment with largest serine acetyltransferase activity of total serine acetyltransferase activity independent of plastidic serine acetyltransferase | Arabidopsis thaliana | 5739 | - |
plastid | SERAT2.1 | Arabidopsis thaliana | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | Arabidopsis thaliana | no major contribution to total cysteine biosynthesis | O-acetylserine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q42538 | At5g56760, SERAT1.1; T-DNA insertion mutants for cytosolic serine acetyltransferase (csat) and plastidic serine acetyltransferase (psat), double mutants of both isoforms (dmsat), and Columbia-0 wild-type | - |
Arabidopsis thaliana | Q42588 | At1g55920 SERAT2.1; T-DNA insertion mutants for cytosolic serine acetyltransferase (csat) and plastidic serine acetyltransferase (psat), double mutants of both isoforms (dmsat), and Columbia-0 wild-type | - |
Purification (Comment) | Organism |
---|---|
frozen plant material is homogenized in extraction buffer (50 mM HEPES/KOH, pH 7.4) and centrifuged, supernatant desalted and used for enzyme assays | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots | Arabidopsis thaliana |
additional information | - |
50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves | Arabidopsis thaliana |
additional information | - |
50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | no major contribution to total cysteine biosynthesis | Arabidopsis thaliana | O-acetylserine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytosolic serine acetyltransferase | - |
Arabidopsis thaliana |
plastidic serine acetyltransferase | - |
Arabidopsis thaliana |
SAT | - |
Arabidopsis thaliana |
SERAT1.1 | - |
Arabidopsis thaliana |
SERAT2.1 | - |
Arabidopsis thaliana |
serine acetyltransferase | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | T-DNA insertion mutants lead to complete expression disruption of the respective enzyme genes and decreases in O-acetyl serine and cysteine contents in leaves and roots (cytosolic enzyme and double mutant), a significant gamma-glutamylcysteine decrease in double mutant leaves and cytosolic enzyme mutant roots, a reduction of glutathione content in cytosolic enzyme mutant roots, and sulfate reduction in cytosolic enzyme mutant leaves | down |
Arabidopsis thaliana | T-DNA insertion mutants lead to complete expression disruption of the respective enzyme genes and decreases in O-acetyl serine and cysteine contents in leaves and roots (double mutant), a significant gamma-glutamylcysteine decrease in double mutant leaves, and sulfide reduction in plastidic enzyme mutant leaves | down |
General Information | Comment | Organism |
---|---|---|
metabolism | rate-limiting step in the cysteine biosynthesis | Arabidopsis thaliana |