Protein Variants | Comment | Organism |
---|---|---|
D287A/D288A | mutation does not substantially change the shape of the kcat-pH profile, suggesting these conserved residues do not function as base catalysts for histone acetylation | Saccharomyces cerevisiae |
D287N | mutation does not substantially change the shape of the kcat-pH profile, suggesting these conserved residues do not function as base catalysts for histone acetylation | Saccharomyces cerevisiae |
D288N | mutation does not substantially change the shape of the kcat-pH profile, suggesting these conserved residues do not function as base catalysts for histone acetylation. Mutant reveals a dramatic 1000fold decrease in kcat/Km for acetyl-CoA | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | CoA binds competitively with acetyl-CoA | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
acetyl-CoA | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.00049 | - |
acetyl-CoA | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0007 | - |
acetyl-CoA | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.007 | - |
histone H3 | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.044 | - |
histone H3 tail peptide | mutant D287A/D288A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.055 | - |
histone H3 tail peptide | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.062 | - |
histone H3 tail peptide | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.112 | - |
histone H3 tail peptide | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q07794 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | sequential mechanism in which acetyl-CoA and H3 bind to a complex of isoform Rtt109 and histone chaperone Vps75 without obligate order, followed by the direct attack of the unprotonated epsilon-amino group on acetyl-CoA, transferring the acetyl-group to H3 lysine residues The chemical attack of substrate lysine on the bound acetyl-CoA is the rate-limiting step of catalysis | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 tail peptide | H3 peptide substrate, amino acid sequence ARTKQTARKSTGGKAPRKQL | Saccharomyces cerevisiae | CoA + acetylhistone H3 peptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isoform Rtt109 and histone chaperone Vps75 form a stable complex with nanomolar binding affinity | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Rtt109 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
histone H3 tail peptide | mutant D287A/D288A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0021 | - |
histone H3 tail peptide | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0028 | - |
acetyl-CoA | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0087 | - |
histone H3 tail peptide | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.012 | - |
acetyl-CoA | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.11 | - |
acetyl-CoA | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.11 | - |
histone H3 tail peptide | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.62 | - |
histone H3 | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
acetyl-CoA | mutant D287A/D288A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.025 | - |
histone H3 tail peptide | mutant D287A/D288A, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.038 | - |
histone H3 tail peptide | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.14 | - |
histone H3 tail peptide | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.6 | - |
acetyl-CoA | mutant D288N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.98 | - |
histone H3 tail peptide | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
17 | - |
acetyl-CoA | mutant D287N, pH 7.5, 25°C | Saccharomyces cerevisiae | |
90 | - |
histone H3 | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
390 | - |
acetyl-CoA | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |