Cloned (Comment) | Organism |
---|---|
gene ARD1, recombinant expression in HEK-293 cells, stable recombinant overexpression of GFP-tagged enzyme in MEF cells, recombinant expression of His-tagged enzyme and of GST-tagged enzyme in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R82A/Y122F | site-directed mutagenesis, the enzyme mutant shows highly reduced lysine acetyltransferase activity with aurora kinase A as substrate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Homo sapiens | 5813 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [AuA]-L-lysine125 | Homo sapiens | lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview | CoA + [AuA]-N6-acetyl-L-lysine125 | - |
? | |
acetyl-CoA + [AuA]-L-lysine75 | Homo sapiens | lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview | CoA + [AuA]-N6-acetyl-L-lysine75 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41227 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme and GST-tagged enzyme from Escherichia coli strain BL21 by nickel and glutathione affinity chromatography, respectively | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | aurora kinase A colocalalizes with ARD1 during cell division and cell migration, aurora A colocalizes with ARD1 at centrosomes during cell division in prophase, metaphase, anaphase, and telophase | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [AuA]-L-lysine125 | lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview | Homo sapiens | CoA + [AuA]-N6-acetyl-L-lysine125 | - |
? | |
acetyl-CoA + [AuA]-L-lysine75 | lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview | Homo sapiens | CoA + [AuA]-N6-acetyl-L-lysine75 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARD1 acetyltransferase | - |
Homo sapiens |
arrest defective protein 1 acetyltransferase | - |
Homo sapiens |
More | see also EC 2.3.1.255 | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | the AuA acetylation level decreases dramatically when ARD1 is mutated at R82 and Y122. The phosphorylation level of AuA is decreased in cells overexpressing K75R/K125R mutant | Homo sapiens |
metabolism | aurora kinase A colocalalizes with ARD1 during cell division and cell migration | Homo sapiens |
physiological function | ARD1-mediated aurora kinase A (AuA) acetylation promotes cell proliferation and migration. ARD1-mediated AuA acetylation at K75/K125 enhances AuA kinase activity. Cells overexpressing AuA double mutant K75R/K125R display a dramatically decreased level of acetylated AuA, suggesting that these sites are critical for the acetylation of AuA by ARD1. AuA interacts with ARD1, and AuA acetylation is regulated by functional ARD1 | Homo sapiens |