General Stability | Organism |
---|---|
DTT stabilizes | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | preincubation of the acetyl transferase with 0.4 mM N-ethylmaleimide results in large decrease of active citrate lyase formed | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Oxidation Stability | Organism |
---|---|
O2-sensitive, DTT stabilizes | Klebsiella aerogenes |
Purification (Comment) | Organism |
---|---|
partial | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.4 | - |
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-acetylphosphopantetheine + deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)] | - |
Klebsiella aerogenes | phosphopantetheine + [citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)] | i.e. active form of EC 4.1.3.6 | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 25% of maximal activity at pH 6, 95% of maximal activity at pH 8 | Klebsiella aerogenes |