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Literature summary for 2.3.2.23 extracted from

  • Hsu, S.H.; Chen, S.H.; Kuo, C.C.; Chang, J.Y.
    Ubiquitin-conjugating enzyme E2 B regulates the ubiquitination of O6-methylguanine-DNA methyltransferase and BCNU sensitivity in human nasopharyngeal carcinoma cells (2018), Biochem. Pharmacol., 158, 327-338 .
    View publication on PubMed
Search for more literature for 2.3.2.23

Activating Compound

Activating Compound Comment Organism Structure
additional information 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and O6-benzylguanine facilitate the ubiquitination and degradation of O6-methylguanine-DNA methyltransferase (MGMT) in several types of cancer cells involving the enzyme ubiquitin-conjugating enzyme E2 B. BCNU enhances the interaction between MGMT and UBE2B Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme UBE2B knockout, HONE-1 and TW01 cells are transfected specific UBE2B siRNA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information analysis of binding affinity between O6-methylguanine-DNA methyltransferase (MGMT)and E2 ubiquitin conjugating enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P63146
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HONE-1 cell
-
 Homo sapiens
-
nasopharyngeal carcinoma cell
-
 Homo sapiens
-
NPC-TW01 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
RAD6B
-
 Homo sapiens
UBE2B
-
 Homo sapiens
ubiquitin-conjugating enzyme E2 B
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction overexpression/knockdown of UBE2B enhances/reduces BCNU-mediated O6-methylguanine-DNA methyltransferase (MGMT) ubiquitination. UBE2B knockdown significantly increases 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU)cytotoxicity in NPC cells. Therefore, loss of UBE2B seems to disrupt ubiquitin-mediated degradation of alkylated MGMT. UBE2B knockdown reduces MGMT activity, suggesting that loss of UBE2B leads to the accumulation of deactivated MGMT and suppresses MGMT protein turnover in BCNU-treated cells Homo sapiens
physiological function ubiquitin-conjugating enzyme E2 B (UBE2B) is a regulator of O6-methylguanine-DNA methyltransferase (MGMT) ubiquitination mediated by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU)in nasopharyngeal carcinoma (NPC) cells. BCNU enhances the interaction between MGMT, RAD18, and ubiquitinated UBE2B. The E3 ubiquitin ligase RAD18, a partner of UBE2B, is also involved in BCNU-mediated MGMT ubiquitination. UBE2B modulates sensitivity to BCNU in NPC cells by regulating MGMT ubiquitination. UBE2B and RAD18 facilitate and accelerate MGMT ubiquitination in vitro, and BCNU and O6-benzylguanine promote UBE2B/RAD18-induced MGMT ubiquitination Homo sapiens