Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Microcystis aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2 mM, 50% residual activity | Microcystis aeruginosa | |
Cu2+ | 2 mM, 12% residual activity | Microcystis aeruginosa | |
Mg2+ | 2 mM, 0.29% residual activity | Microcystis aeruginosa | |
Mn2+ | 2 mM, 72% residual activity | Microcystis aeruginosa | |
Ni2+ | 2 mM, 71% residual activity | Microcystis aeruginosa | |
Zn2+ | 2 mM, 27% residual activity | Microcystis aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
acetyl-CoA | pH 8.0, 25°C | Microcystis aeruginosa | |
0.103 | - |
oxaloacetate | pH 8.0, 25°C | Microcystis aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microcystis aeruginosa | A8YCJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Microcystis aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Microcystis aeruginosa | citrate + CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Microcystis aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
20 min, 11% loss of activity | Microcystis aeruginosa |
55 | - |
half-life about 35 min | Microcystis aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.1 | - |
acetyl-CoA | pH 8.0, 25°C | Microcystis aeruginosa | |
24.5 | - |
oxaloacetate | pH 8.0, 25°C | Microcystis aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Microcystis aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
238 | - |
oxaloacetate | pH 8.0, 25°C | Microcystis aeruginosa | |
1474 | - |
acetyl-CoA | pH 8.0, 25°C | Microcystis aeruginosa |