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Literature summary for 2.3.3.19 extracted from
- Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293 (1988), J. Antibiot., 41, 1057-1065.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.05 mM, no residual activity. Activtity can be completely restored by addition of Co2+, or Mn2+ | Streptomyces hygroscopicus |  |
| iodoacetamide | 1 mM, 18% residual activity | Streptomyces hygroscopicus | |
| p-chloromercuribenzoate | 0.1 mM, 2% residual activity | Streptomyces hygroscopicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
acetyl-CoA | pH 7.5, 30°C | Streptomyces hygroscopicus | |
| 0.13 | - |
oxalacetic acid | pH 7.5, 30°C | Streptomyces hygroscopicus | |
| 0.39 | - |
3-phosphonopyruvate | pH 7.5, 30°C | Streptomyces hygroscopicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | addition completeley restores inactivation by EDTA | Streptomyces hygroscopicus | |
| Mn2+ | addition completeley restores inactivation by EDTA | Streptomyces hygroscopicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
| 90000 | 98000 | gel filtration | Streptomyces hygroscopicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus | Q9LCB4 | - |
- |
| Streptomyces hygroscopicus SF-1293 | Q9LCB4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from cell-free extract | Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + 3-phosphonopyruvate | - |
Streptomyces hygroscopicus | (R)-2-(phosphonomethyl)malate + CoA | - |
? | |
| acetyl-CoA + H2O + 3-phosphonopyruvate | - |
Streptomyces hygroscopicus SF-1293 | (R)-2-(phosphonomethyl)malate + CoA | - |
? | |
| acetyl-CoA + H2O + oxalacetic acid | - |
Streptomyces hygroscopicus | ? + CoA | - |
? | |
| additional information | no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate | Streptomyces hygroscopicus | ? | - |
? | |
| additional information | no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate | Streptomyces hygroscopicus SF-1293 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces hygroscopicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces hygroscopicus |
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