Literature summary for 2.3.3.21 extracted from

Risso, C.; Van Dien, S.J.; Orloff, A.; Lovley, D.R.; Coppi, M.V.
Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens (2008), J. Bacteriol., 190, 2266-2274.

Search for more literature for 2.3.3.21

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + pyruvate + H2O	Geobacter sulfurreducens	-	CoA + (2S)-2-hydroxy-2-methylbutanedioate	-	r

Organism

Organism	UniProt	Comment	Textmining
Geobacter sulfurreducens	Q74C76	2-isopropylmalate synthase	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0004	-	strain DLCR6, cimA::Gmr	Geobacter sulfurreducens
0.0004	-	strain DLCR7, tdcB::Knr cimA::Gmr	Geobacter sulfurreducens
0.0051	-	strain DLCR5, tdcB::Knr	Geobacter sulfurreducens
0.0074	-	strain DL1, wild type	Geobacter sulfurreducens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + pyruvate + H2O	-	Geobacter sulfurreducens	CoA + (2S)-2-hydroxy-2-methylbutanedioate	-	r

Synonyms

Synonyms	Comment	Organism
CimA	-	Geobacter sulfurreducens
citramalate lyase	-	Geobacter sulfurreducens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	enzyme assay	Geobacter sulfurreducens

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Release 2023.1 (January 2023)