Application | Comment | Organism |
---|---|---|
synthesis | enzyme is an important biocatalyst for production of fructose homopolymers, potential usage in bioindustrial fields | Rahnella aquatilis |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli DH5alpha | Rahnella aquatilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Zymomonas mobilis |
additional information | - |
amino acid sequence alignment | Rahnella aquatilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rahnella aquatilis | O54435 | strain ATCC33071, gene lsrA | - |
Rahnella aquatilis ATCC 33071 | O54435 | strain ATCC33071, gene lsrA | - |
Zymomonas mobilis | - |
gene levU | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli as His-tagged protein | Rahnella aquatilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats | Zymomonas mobilis | |
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats | Rahnella aquatilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | transfucosylation from sucrose to various acceptors, except sugar alcohols | Rahnella aquatilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | transfucosylation from sucrose to various acceptors, except sugar alcohols | Rahnella aquatilis ATCC 33071 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis ATCC 33071 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zymomonas mobilis |
additional information | - |
the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis |
- |
- |
- |
Zymomonas mobilis |
20 | - |
levan production | Rahnella aquatilis |
40 | - |
levan formation | Rahnella aquatilis |
50 | - |
- |
Zymomonas mobilis |
50 | - |
maximal reaction velocity | Rahnella aquatilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Zymomonas mobilis |
4 | 6 | recombinant enzyme | Rahnella aquatilis |