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show all sequences of 2.4.1.10

Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium

Homann, A.; Biedendieck, R.; Götze, S.; Jahn, D.; Seibel, J.; Biochem. J. 407, 189-198 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; wild-type enzyme and 16 variants are expressed in Escherichia coli
Bacillus megaterium
Engineering
Amino acid exchange
Commentary
Organism
D257A
mutant shows almost no activity; site-directed mutagenesis, no activity
Bacillus megaterium
D95A
site-directed mutagenesis, no activity
Bacillus megaterium
E350A
mutant is nearly inactive; site-directed mutagenesis, nearly inactive
Bacillus megaterium
E352A
site-directed mutagenesis, no measureable activity
Bacillus megaterium
L118A
kcat/Km is 5.5% of wild-type value; site-directed mutagenesis
Bacillus megaterium
N252A
kcat/Km is 104% of wild-type value; site-directed mutagenesis
Bacillus megaterium
R256A
mutant is nearly inactive; site-directed mutagenesis, nearly inactive
Bacillus megaterium
R370A
site-directed mutagenesis, after a reaction time of 60 min an accumulation of neokestose (2,6-beta-Fru-betaGlc-1,2-beta-Fru, 32.7 mM) is determined, whereas after 19 h, blastose (2,6-beta-Fru-alpha,betaGlc) is the main reaction product (69.7 mM)
Bacillus megaterium
S173A
kcat/Km is 46% of wild-type value; site-directed mutagenesis
Bacillus megaterium
W172A
kcat/Km is 1.4% of wild-type value; site-directed mutagenesis, 72fold increase of the KM of the wild-type
Bacillus megaterium
W94A
kcat/Km is 9% of wild-type value; site-directed mutagenesis, 9% of the catalytic efficiency of the wild-type
Bacillus megaterium
Y421A
kcat/Km is 1.9% of wild-type value; site-directed mutagenesis, 3%of the wild-type activity
Bacillus megaterium
General Stability
General Stability
Organism
in standard reactions, long-term temperature treatment revealed a high protein stability at 37°C for at least 24h
Bacillus megaterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
2.3 mM (sucrose) total, mutant enzyme S173A; 29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site; 31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type; 35.1 mM (sucrose) total, mutant enzyme N252H; 4.1 mM (sucrose) total, mutant enzyme N252A; 480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type; 51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity; 66.6 mM (sucrose), mutant enzyme L118A; 6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensen’s phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min; 7.5 mM (sucrose) total, mutant enzyme N252G; 8.4 mM (sucrose) total, mutant enzyme N252D; mutant enzyme D257A, no activity; mutant enzyme D95A, no activity; mutant enzyme E350A, nearly inactive; mutant enzyme E352A, no measureable activity; mutant enzyme R256A, nearly inactive
Bacillus megaterium
2.3
-
sucrose
pH 6.6, mutant enzyme S173A
Bacillus megaterium
4.1
-
sucrose
pH 6.6, mutant enzyme N252A
Bacillus megaterium
6.6
-
sucrose
pH 6.6, wild-type enzyme
Bacillus megaterium
31.9
-
sucrose
pH 6.6, mutant enzyme W94A
Bacillus megaterium
51.9
-
sucrose
pH 6.6, mutant enzyme Y421A
Bacillus megaterium
66.6
-
sucrose
pH 6.6, mutant enzyme L118A
Bacillus megaterium
480.4
-
sucrose
pH 6.6, mutant enzyme W172A
Bacillus megaterium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus megaterium
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
FPLC, proteins are identified by peptide mass fingerprinting, as well as by using postsource decay fragmentation data recorded with an Ultraflex MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometer; MALDI-TOF
Bacillus megaterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
DSM319; strain DSM319, gene sacB, expression in Escherichia coli, 74% identity at the amino acid sequence level with SacB from Bacillus subtilis
-
Bacillus megaterium DSM 319
-
DSM319; strain DSM319, gene sacB, expression in Escherichia coli, 74% identity at the amino acid sequence level with SacB from Bacillus subtilis
-
Purification (Commentary)
Commentary
Organism
; by FPLC using a 15 ml CM-Sepharose column system
Bacillus megaterium
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Bacillus megaterium
-
Storage Stability
Storage Stability
Organism
-20°C, pH 6.6, stable for more than 6 months
Bacillus megaterium
20°C, more than 6 months, pH 6.6
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sucrose + (2,6-beta-D-fructosyl)n
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
678072
Bacillus megaterium
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
678072
Bacillus megaterium DSM 319
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + ?
-
678072
Bacillus megaterium
blastose + ?
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
-
?
sucrose + ?
-
678072
Bacillus megaterium DSM 319
blastose + ?
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
-
?
sucrose + ?
-
678072
Bacillus megaterium
polyfructan + ?
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
-
?
sucrose + ?
-
678072
Bacillus megaterium DSM 319
polyfructan + ?
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + 1-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium DSM 319
D-glucose + 1-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + 6-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium DSM 319
D-glucose + 6-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + neokestose
identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + nystose
identified by HPAEC
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
; wild-type, pH6.6, 50 mM Sorensen’s buffer
Bacillus megaterium
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
high stability for at least 24 h
Bacillus megaterium
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
325
-
sucrose
pH 6.6, mutant enzyme Y421A
Bacillus megaterium
335
-
sucrose
mutant enzyme Y421A
Bacillus megaterium
363
-
sucrose
mutant enzyme S173A; pH 6.6, mutant enzyme S173A
Bacillus megaterium
1000
-
sucrose
mutant enzyme W94A; pH 6.6, mutant enzyme W94A
Bacillus megaterium
1231
-
sucrose
mutant enzyme L118A; pH 6.6, mutant enzyme L118A
Bacillus megaterium
1480
-
sucrose
mutant enzyme N252A; pH 6.6, mutant enzyme N252A
Bacillus megaterium
1529
-
sucrose
mutant enzyme N252H
Bacillus megaterium
2256
-
sucrose
mutant enzyme N252G
Bacillus megaterium
2272
-
sucrose
pH 6.6, wild-type enzyme; wild-type
Bacillus megaterium
2396
-
sucrose
mutant enzyme W172A; pH 6.6, mutant enzyme W172A
Bacillus megaterium
3743
-
sucrose
mutant enzyme N252D
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
; wild-type, 50 mM Sorensen’s phosphate buffer at 37°C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
Bacillus megaterium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.6
7.6
the enzyme activity significantly decreases at pH values below 5.6 and above 7.6; wild-type, 50 mM Sorensen’s phosphate buffer at 37°C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; wild-type enzyme and 16 variants are expressed in Escherichia coli
Bacillus megaterium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D257A
mutant shows almost no activity; site-directed mutagenesis, no activity
Bacillus megaterium
D95A
site-directed mutagenesis, no activity
Bacillus megaterium
E350A
mutant is nearly inactive; site-directed mutagenesis, nearly inactive
Bacillus megaterium
E352A
site-directed mutagenesis, no measureable activity
Bacillus megaterium
L118A
kcat/Km is 5.5% of wild-type value; site-directed mutagenesis
Bacillus megaterium
N252A
kcat/Km is 104% of wild-type value; site-directed mutagenesis
Bacillus megaterium
R256A
mutant is nearly inactive; site-directed mutagenesis, nearly inactive
Bacillus megaterium
R370A
site-directed mutagenesis, after a reaction time of 60 min an accumulation of neokestose (2,6-beta-Fru-betaGlc-1,2-beta-Fru, 32.7 mM) is determined, whereas after 19 h, blastose (2,6-beta-Fru-alpha,betaGlc) is the main reaction product (69.7 mM)
Bacillus megaterium
S173A
kcat/Km is 46% of wild-type value; site-directed mutagenesis
Bacillus megaterium
W172A
kcat/Km is 1.4% of wild-type value; site-directed mutagenesis, 72fold increase of the KM of the wild-type
Bacillus megaterium
W94A
kcat/Km is 9% of wild-type value; site-directed mutagenesis, 9% of the catalytic efficiency of the wild-type
Bacillus megaterium
Y421A
kcat/Km is 1.9% of wild-type value; site-directed mutagenesis, 3%of the wild-type activity
Bacillus megaterium
General Stability (protein specific)
General Stability
Organism
in standard reactions, long-term temperature treatment revealed a high protein stability at 37°C for at least 24h
Bacillus megaterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
2.3 mM (sucrose) total, mutant enzyme S173A; 29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site; 31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type; 35.1 mM (sucrose) total, mutant enzyme N252H; 4.1 mM (sucrose) total, mutant enzyme N252A; 480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type; 51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity; 66.6 mM (sucrose), mutant enzyme L118A; 6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensen’s phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min; 7.5 mM (sucrose) total, mutant enzyme N252G; 8.4 mM (sucrose) total, mutant enzyme N252D; mutant enzyme D257A, no activity; mutant enzyme D95A, no activity; mutant enzyme E350A, nearly inactive; mutant enzyme E352A, no measureable activity; mutant enzyme R256A, nearly inactive
Bacillus megaterium
2.3
-
sucrose
pH 6.6, mutant enzyme S173A
Bacillus megaterium
4.1
-
sucrose
pH 6.6, mutant enzyme N252A
Bacillus megaterium
6.6
-
sucrose
pH 6.6, wild-type enzyme
Bacillus megaterium
31.9
-
sucrose
pH 6.6, mutant enzyme W94A
Bacillus megaterium
51.9
-
sucrose
pH 6.6, mutant enzyme Y421A
Bacillus megaterium
66.6
-
sucrose
pH 6.6, mutant enzyme L118A
Bacillus megaterium
480.4
-
sucrose
pH 6.6, mutant enzyme W172A
Bacillus megaterium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus megaterium
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
FPLC, proteins are identified by peptide mass fingerprinting, as well as by using postsource decay fragmentation data recorded with an Ultraflex MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectrometer; MALDI-TOF
Bacillus megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
; by FPLC using a 15 ml CM-Sepharose column system
Bacillus megaterium
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Bacillus megaterium
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 6.6, stable for more than 6 months
Bacillus megaterium
20°C, more than 6 months, pH 6.6
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sucrose + (2,6-beta-D-fructosyl)n
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
678072
Bacillus megaterium
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
678072
Bacillus megaterium DSM 319
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + ?
-
678072
Bacillus megaterium
blastose + ?
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
-
?
sucrose + ?
-
678072
Bacillus megaterium DSM 319
blastose + ?
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
-
?
sucrose + ?
-
678072
Bacillus megaterium
polyfructan + ?
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
-
?
sucrose + ?
-
678072
Bacillus megaterium DSM 319
polyfructan + ?
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + 1-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium DSM 319
D-glucose + 1-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + 6-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium DSM 319
D-glucose + 6-kestose
identified by HPAEC
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + neokestose
identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
-
-
?
sucrose + sucrose
-
678072
Bacillus megaterium
D-glucose + nystose
identified by HPAEC
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
; wild-type, pH6.6, 50 mM Sorensen’s buffer
Bacillus megaterium
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
high stability for at least 24 h
Bacillus megaterium
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
325
-
sucrose
pH 6.6, mutant enzyme Y421A
Bacillus megaterium
335
-
sucrose
mutant enzyme Y421A
Bacillus megaterium
363
-
sucrose
mutant enzyme S173A; pH 6.6, mutant enzyme S173A
Bacillus megaterium
1000
-
sucrose
mutant enzyme W94A; pH 6.6, mutant enzyme W94A
Bacillus megaterium
1231
-
sucrose
mutant enzyme L118A; pH 6.6, mutant enzyme L118A
Bacillus megaterium
1480
-
sucrose
mutant enzyme N252A; pH 6.6, mutant enzyme N252A
Bacillus megaterium
1529
-
sucrose
mutant enzyme N252H
Bacillus megaterium
2256
-
sucrose
mutant enzyme N252G
Bacillus megaterium
2272
-
sucrose
pH 6.6, wild-type enzyme; wild-type
Bacillus megaterium
2396
-
sucrose
mutant enzyme W172A; pH 6.6, mutant enzyme W172A
Bacillus megaterium
3743
-
sucrose
mutant enzyme N252D
Bacillus megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
; wild-type, 50 mM Sorensen’s phosphate buffer at 37°C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
Bacillus megaterium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.6
7.6
the enzyme activity significantly decreases at pH values below 5.6 and above 7.6; wild-type, 50 mM Sorensen’s phosphate buffer at 37°C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
Bacillus megaterium
Other publictions for EC 2.4.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735834
Ishida
Levansucrase from Leuconostoc ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides NTM048
Biotechnol. Lett.
38
681-687
2016
-
-
1
-
-
-
10
-
-
3
-
2
-
6
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
-
3
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735552
Li
Recent novel applications of l ...
Bacillus licheniformis, Bacillus subtilis, Microbacterium laevaniformans
Appl. Microbiol. Biotechnol.
99
6959-6969
2015
-
3
-
-
-
-
-
-
3
-
-
3
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
735800
Li
Purification and characterizat ...
Bacillus velezensis, Bacillus velezensis SK 21.002
Biotechnol. Appl. Biochem.
62
815-822
2015
-
-
-
-
-
-
7
2
2
2
1
2
-
4
-
-
1
-
-
-
1
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
2
2
1
2
-
-
-
1
-
-
1
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
735857
Mehmood
Expression of extra-cellular l ...
Pseudomonas savastanoi pv. glycinea, Pseudomonas savastanoi pv. glycinea PG4180
BMC Microbiol.
15
48
2015
-
-
1
-
-
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
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2
3
3
2
-
-
735888
Porras-Dominguez
Bacillus subtilis 168 levansuc ...
Bacillus subtilis 168, Bacillus subtilis
Carbohydr. Polym.
132
338-344
2015
-
-
1
-
-
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-
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-
2
-
4
-
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4
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1
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1
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-
-
-
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-
735890
Li
Bacillus amyloliquefaciens lev ...
Bacillus amyloliquefaciens
Carbohydr. Polym.
133
203-212
2015
-
1
-
-
-
-
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2
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4
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1
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1
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-
-
-
735894
Wu
Biosynthesis of lactosylfructo ...
Bacillus velezensis, Bacillus velezensis SK 21.002
Carbohydr. Res.
401
122-126
2015
-
-
-
-
-
-
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-
2
-
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4
-
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1
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1
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2
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1
1
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1
1
-
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-
-
-
-
-
-
736295
Li
Efficient biosynthesis of lact ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B?512
J. Agric. Food Chem.
63
9755-9763
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
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1
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1
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1
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-
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-
736648
Santos-Moriano
-
Levan versus fructooligosaccha ...
Zymomonas mobilis
J. Mol. Catal. B
119
18-25
2015
-
-
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-
-
-
-
-
-
1
-
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1
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1
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1
1
1
1
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1
1
-
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-
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-
-
-
-
736704
Wuerges
The crystal structure of Erwin ...
Erwinia amylovora, Erwinia amylovora ATCC 49946
J. Struct. Biol.
191
290-298
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
9
-
-
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1
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1
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-
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-
-
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-
3
3
-
-
-
736891
Visnapuu
Levansucrases of a Pseudomonas ...
Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis, Burkholderia cepacia, Dactylis glomerata, Erwinia amylovora, Gluconacetobacter diazotrophicus, Lactobacillus gasseri, Lactobacillus panis, Lactobacillus reuteri, Lactobacillus sanfranciscensis, Phleum pratense, Pseudomonas chlororaphis subsp. aurantiaca, Pseudomonas syringae, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000, Zymomonas mobilis
New Biotechnol.
32
597-605
2015
-
-
-
2
35
-
2
4
16
-
-
-
-
21
-
-
-
-
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38
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6
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3
3
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3
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2
35
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3
3
4
17
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38
-
6
-
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3
4
-
-
-
-
19
21
-
-
-
730222
Tian
-
Optimization of levansucrase/e ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens ATCC 23350
J. Mol. Catal. B
109
85-93
2014
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
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2
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1
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1
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-
-
-
736695
Rasmussen
Transcript profiling of fructa ...
Lolium perenne
J. Plant Physiol.
171
475-485
2014
-
1
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-
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-
-
-
-
-
-
1
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736849
Mardo
High-throughput assay of levan ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Molecules
19
8434-8455
2014
-
1
1
-
31
-
-
-
1
-
-
2
-
6
-
-
-
-
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2
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1
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-
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1
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1
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-
31
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2
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2
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4
1
1
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1
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2
2
-
-
-
737179
Lu
-
A recombinant levansucrase fro ...
Bacillus licheniformis, Bacillus licheniformis 8-37-0-1
Process Biochem.
49
1503-1510
2014
-
-
1
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
-
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1
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10
1
1
1
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1
1
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1
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1
1
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1
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1
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1
-
10
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
736130
Vigants
An influence of ethanol and te ...
Zymomonas mobilis 113S, Zymomonas mobilis
Folia Microbiol. (Praha)
58
75-80
2013
1
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
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2
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1
-
-
1
-
-
-
-
1
1
-
-
-
736970
Verspreet
Fructan metabolism in developi ...
Triticum aestivum
Plant Cell Physiol.
54
2047-2057
2013
-
-
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-
-
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-
-
-
-
-
-
-
2
-
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1
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1
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2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704960
Rairakhwada
Gene cloning, characterization ...
Bacillus amyloliquefaciens
J. Ind. Microbiol. Biotechnol.
37
195-204
2010
-
-
1
-
-
-
-
2
2
-
1
2
-
6
-
-
1
-
-
-
3
-
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1
2
1
-
-
2
1
-
-
-
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1
-
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2
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1
2
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1
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3
-
2
1
2
1
-
-
2
1
-
-
-
-
-
-
-
-
701567
El-Refai
-
Immobilization and properties ...
Bacillus circulans
Acta Pharm. Sci.
51
149-156
2009
-
-
-
-
1
-
5
1
1
1
-
-
-
1
-
-
1
-
-
-
1
-
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1
1
3
-
2
1
1
-
-
-
-
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-
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1
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5
-
1
1
1
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-
-
1
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1
-
-
-
1
1
3
-
2
1
1
-
-
-
-
-
-
-
701787
Menendez
Transcriptional regulation and ...
Gluconacetobacter diazotrophicus
Appl. Environ. Microbiol.
75
1782-1785
2009
-
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701837
Chiang
Enhanced levan production usin ...
Zymomonas mobilis
Appl. Microbiol. Biotechnol.
82
445-451
2009
-
1
1
-
1
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
701838
Kang
Synthesis and characterization ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
Appl. Microbiol. Biotechnol.
83
1009-1016
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702880
Ortiz-Soto
Evaluation of cross-linked agg ...
Bacillus subtilis
BMC Biotechnol.
9
68-75
2009
-
-
-
-
3
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703493
Nam
-
Synthesis, structural analysis ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
Enzyme Microb. Technol.
45
247-252
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
705086
Han
Production of lactosucrose fro ...
Zymomonas mobilis
J. Microbiol. Biotechnol.
19
1153-1160
2009
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
706683
Visnapuu
Fully automated chip-based neg ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Rapid Commun. Mass Spectrom.
23
1337-1346
2009
-
-
1
-
-
-
-
-
-
-
-
4
-
8
-
-
1
-
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706915
El-Refai
-
Enzymic synthesis of levan and ...
Bacillus circulans
World J. Microbiol. Biotechnol.
25
821-827
2009
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
1
-
3
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
3
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
684132
Li
Amino acid substitutions of Hi ...
Zymomonas mobilis
Acta Biochim. Pol.
55
201-206
2008
-
-
1
-
10
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684765
Waldherr
Genetic and functional charact ...
Lactobacillus panis
Arch. Microbiol.
190
497-505
2008
-
-
1
-
-
-
1
1
-
1
-
1
-
2
-
-
1
-
-
-
-
-
6
-
1
3
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
3
-
-
1
1
-
-
-
-
-
-
-
-
685846
Meng
Donor substrate recognition in ...
Bacillus subtilis
BMC Struct. Biol.
8
16
2008
-
-
1
1
3
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687775
Goldman
Two active forms of Zymomonas ...
Zymomonas mobilis
J. Biol. Chem.
283
32209-32217
2008
-
-
1
-
1
-
1
3
1
-
2
-
-
2
-
-
1
-
-
-
-
-
3
2
-
-
-
3
-
1
-
-
3
-
-
-
-
1
-
-
1
-
-
1
3
3
1
-
2
-
-
-
-
1
-
-
-
-
3
2
-
-
-
3
-
1
-
-
-
-
-
-
-
-
689854
Ortiz-Soto
Selected mutations in Bacillus ...
Bacillus subtilis
Protein Eng. Des. Sel.
21
589-595
2008
2
-
1
1
13
-
-
10
-
-
-
-
-
3
-
-
1
-
-
-
-
-
4
-
-
-
2
10
-
-
-
-
-
-
-
2
-
1
-
1
13
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
2
10
-
-
-
-
-
-
-
-
-
-
701566
Abdel-fattah
-
Biosynthesis of levansucrase b ...
Bacillus circulans
Acta Pharm. Sci.
50
203-212
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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1
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702972
Esawy
-
Immobilisation of Bacillus sub ...
Bacillus subtilis, Bacillus subtilis NRC33a
Braz. J. Chem. Eng.
25
237-246
2008
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8
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1
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671551
Biedendieck
Export, purification, and acti ...
Lactobacillus reuteri, Lactobacillus reuteri 21
Appl. Microbiol. Biotechnol.
74
1062-1073
2007
-
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1
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6
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678072
Homann
Insights into polymer versus o ...
Bacillus megaterium, Bacillus megaterium DSM 319
Biochem. J.
407
189-198
2007
-
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1
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1
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9
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12
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11
1
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688315
Kang
Heterologous expression and op ...
Pseudomonas chlororaphis subsp. aurantiaca, Pseudomonas chlororaphis subsp. aurantiaca S-4380
J. Microbiol. Biotechnol.
17
1751-1757
2007
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689722
Szwengiel
-
Levan synthesis during associa ...
Bacillus subtilis
Pol. J. Food Nutr. Sci.
57
433-440
2007
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1
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671449
Malten
A Bacillus megaterium plasmid ...
Lactobacillus reuteri 121, Lactobacillus reuteri
Appl. Environ. Microbiol.
72
1677-1679
2006
-
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672929
Seibel
Synthesis of sucrose analogues ...
Bacillus subtilis 168, Bacillus subtilis
Carbohydr. Res.
341
2335-2349
2006
-
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1
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1
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673743
Li
Thermo-responsive expression a ...
Pseudomonas syringae
FEMS Microbiol. Lett.
265
178-185
2006
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675816
van Hijum
Structure-function relationshi ...
Bacillus subtilis, Gluconacetobacter diazotrophicus, Lactobacillus sp., Streptococcus salivarius, Streptococcus sp., Zymomonas mobilis
Microbiol. Mol. Biol. Rev.
70
157-176
2006
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675837
Ozimek
The levansucrase and inulosucr ...
Lactobacillus reuteri 121, Lactobacillus reuteri
Microbiology
152
1187-1196
2006
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675845
Laue
Contribution of alginate and l ...
Pseudomonas syringae
Microbiology
152
2909-2918
2006
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658750
Ozimek
Mutational analysis of the rol ...
Lactobacillus reuteri 121, Lactobacillus reuteri
FEBS Lett.
579
1124-1128
2005
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13
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671507
Tieking
Molecular and functional chara ...
Lactobacillus sanfranciscensis, Lactobacillus sanfranciscensis TMW 1.392
Appl. Microbiol. Biotechnol.
66
655-663
2005
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1
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1
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2
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6
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1
1
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1
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671836
Martinez-Fleites
Crystal structure of levansucr ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
Biochem. J.
390
19-27
2005
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672335
Kang
Cloning and expression of leva ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
Biochim. Biophys. Acta
1727
5-15
2005
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1
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672734
Park
Lactosucrose production by var ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens IFO15535, Bacillus subtilis, Geobacillus stearothermophilus, Geobacillus stearothermophilus ATCC 12980, Paenibacillus polymyxa, Paenibacillus polymyxa KCCM35411, Pseudomonas syringae, Pseudomonas syringae IFO14086, Rahnella aquatilis, Rahnella aquatilis KCTC2858, Sterigmatomyces elviae, Sterigmatomyces elviae ATCC 18894
Biotechnol. Lett.
27
495-497
2005
-
7
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673208
Abdel-Fattah
Production of levansucrase fro ...
Bacillus subtilis, Bacillus subtilis NRC 33a
Curr. Microbiol.
51
402-407
2005
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4
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675733
Daguer
Increasing the stability of sa ...
Bacillus subtilis
Lett. Appl. Microbiol.
41
221-226
2005
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1
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-
488326
Kim
-
Enzymatic characterization of ...
Rahnella aquatilis, Rahnella aquatilis ATCC 15552
J. Microbiol. Biotechnol.
13
230-235
2003
-
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1
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2
1
1
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1
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488329
Ben Ammar
Characterization of a thermost ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TH4-2
J. Biotechnol.
99
111-119
2002
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1
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1
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1
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2
1
2
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1
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1
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488343
Yanase
Identification of functionally ...
Zymomonas mobilis
J. Biochem.
132
565-572
2002
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1
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15
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2
-
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1
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15
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3
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2
1
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-
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488327
Trujillo
Fructo-oligosaccharides produc ...
Gluconacetobacter diazotrophicus
Enzyme Microb. Technol.
28
139-144
2001
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1
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2
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6
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488342
Bergeron
Roles of fructosyltransferase ...
Actinomyces naeslundii, Actinomyces naeslundii WVU45
Infect. Immun.
69
5395-5402
2001
-
-
1
-
2
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-
-
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-
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1
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1
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488344
Jang
-
Comparison of characteristics ...
Zymomonas mobilis
Biotechnol. Lett.
23
339-344
2001
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1
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488328
Gon Kim
Synthesis of methyl beta-D-fru ...
Rahnella aquatilis
Enzyme Microb. Technol.
27
646-651
2000
-
-
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-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
488341
Sunitha
Refolding and purification of ...
Zymomonas mobilis, Zymomonas mobilis ZM1
Protein Expr. Purif.
18
388-393
2000
-
-
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-
-
-
-
-
2
-
1
-
-
5
-
-
1
-
1
-
-
-
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1
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
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1
-
-
-
2
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
488338
Leloup
Differential dependence of lev ...
Bacillus subtilis
J. Bacteriol.
181
1820-1826
1999
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
488339
Hernandez
Levansucrase from Acetobacter ...
Gluconacetobacter diazotrophicus
Curr. Microbiol.
39
146-152
1999
1
-
1
-
-
-
4
-
2
-
1
1
-
4
-
-
1
-
-
-
2
-
2
1
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
1
1
-
-
-
1
-
-
2
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488340
Batista
-
Substitution of Asp-309 by Asn ...
Gluconacetobacter diazotrophicus
Biochem. J.
337
503-506
1999
-
-
1
-
1
-
-
2
1
-
1
-
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
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488331
Song
Levansucrase of Rahnella aquat ...
Rahnella aquatilis, Rahnella aquatilis ATCC 33071, Zymomonas mobilis
Ann. N. Y. Acad. Sci.
864
506-511
1998
-
1
1
-
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2
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4
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-
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-
-
488332
Euzenat
-
Production and purification of ...
Bacillus subtilis
Ann. N. Y. Acad. Sci.
864
288-294
1998
1
-
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-
1
1
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1
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1
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-
-
-
-
-
-
-
488337
Hochstrasser
Expression of a functional bar ...
Hordeum vulgare
FEBS Lett.
440
356-360
1998
-
-
1
-
1
-
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-
-
-
1
-
5
-
-
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-
-
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-
488336
Sprenger
Fructan synthesis in transgeni ...
Hordeum vulgare
FEBS Lett.
400
355-358
1997
-
-
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-
1
-
-
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1
-
5
-
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-
-
-
-
-
-
-
488335
Perez Oseguera
-
Properties of levansucrase fro ...
Bacillus circulans
Appl. Microbiol. Biotechnol.
45
465-471
1996
1
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
1
-
-
-
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1
2
-
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1
-
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-
-
7
1
2
-
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2
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-
-
-
-
-
488330
Hettwer
Purification and characterizat ...
Bacillus subtilis, Erwinia amylovora, Pseudomonas savastanoi pv. phaseolicola, Streptococcus mutans, Zymomonas mobilis
J. Bacteriol.
177
2834-2839
1995
2
-
-
-
-
1
9
1
4
-
7
-
-
12
-
-
1
-
-
-
1
1
4
2
2
1
-
-
2
1
-
-
-
-
-
2
-
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-
-
1
-
9
-
1
4
-
7
-
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-
1
-
-
1
1
4
2
2
1
-
-
2
1
-
-
-
-
-
-
-
-
488333
Sprenger
Purification, cloning, and fun ...
Hordeum vulgare
Proc. Natl. Acad. Sci. USA
92
11652-11656
1995
-
-
1
-
1
-
-
-
-
-
4
2
-
4
-
-
1
-
-
-
-
-
3
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1
-
-
-
1
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-
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1
1
-
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1
-
-
-
-
-
-
-
-
-
488334
Duchateau
Sucrose:fructan 6-fructosyltra ...
Hordeum vulgare
Plant Physiol.
107
1249-1255
1995
-
-
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-
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1
-
2
-
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1
-
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-
3
1
1
-
-
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1
-
-
-
-
-
-
-
-
-
488345
Hernandez
-
Isolation and enzymic properti ...
Gluconacetobacter diazotrophicus
Biochem. J.
309
113-118
1995
-
-
-
-
-
-
2
-
1
1
2
1
-
1
-
-
1
1
-
-
-
-
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1
1
-
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1
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-
-
-
1
-
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-
4
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
488312
Yanase
-
Purification, crystallization, ...
Zymomonas mobilis
Biosci. Biotechnol. Biochem.
56
1335-1337
1992
-
-
-
1
-
-
5
1
1
-
2
-
-
1
-
-
1
-
-
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1
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-
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1
1
-
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-
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-
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-
-
-
5
-
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2
-
-
-
-
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-
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-
-
3
1
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-
1
-
1
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1
-
-
-
-
-
-
-
488313
Ohtsuka
-
Characterization of levansucra ...
Rahnella aquatilis, Rahnella aquatilis JCM -1683
Biosci. Biotechnol. Biochem.
56
1373-1377
1992
-
-
-
-
-
-
3
1
-
-
2
-
-
2
-
-
1
-
-
-
1
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15
1
3
-
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-
1
1
1
-
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-
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-
3
-
1
1
1
-
-
-
-
-
-
-
488325
Chambert
-
Polymerase and hydrolase activ ...
Bacillus subtilis
Biochem. J.
279
35-41
1991
-
-
1
-
5
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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-
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-
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-
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-
4
-
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-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
488310
Chambert
Reversible thermal unfolding o ...
Bacillus subtilis, Bacillus subtilis QB112
FEBS Lett.
275
61-64
1990
-
-
-
-
-
1
-
-
-
2
-
-
-
3
-
-
-
-
1
-
-
-
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
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2
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
488309
Cote
-
Purification and properties of ...
Pantoea agglomerans
Carbohydr. Res.
190
299-307
1989
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
-
-
-
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1
-
-
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-
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-
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-
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-
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-
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-
-
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-
-
-
-
1
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
488324
Sauerstein
Detection and characterization ...
Pseudomonas savastanoi pv. phaseolicola
J. Basic Microbiol.
28
667-672
1988
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
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-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
488316
Yamamoto
-
The mode of synthesis of levan ...
Bacillus subtilis
Agric. Biol. Chem.
49
343-349
1985
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488317
Fouet
Characterization of the precur ...
Bacillus subtilis
Biochem. Biophys. Res. Commun.
119
795-800
1984
-
-
1
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
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1
-
-
-
-
-
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-
-
-
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-
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-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488314
Lyness
-
Levansucrase from Zymomonas mo ...
Zymomonas mobilis
Biotechnol. Lett.
5
345-350
1983
3
-
-
-
-
-
6
1
-
1
1
2
-
1
-
-
1
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
6
-
1
-
1
1
2
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488321
Mäntsälä
-
Comparison of levansucrase fro ...
Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus subtilis QB127
FEMS Microbiol. Lett.
13
395-399
1982
-
-
-
-
-
-
2
2
4
-
6
3
-
3
-
-
-
-
-
-
2
-
6
2
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
4
-
6
3
-
-
-
-
-
-
2
-
6
2
2
-
2
-
2
-
-
-
-
-
-
-
-
-
488318
LeBrun
The structure of Bacillus subt ...
Bacillus subtilis
J. Biol. Chem.
255
12034-12036
1980
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488320
Tanaka
Synthesis of levan by levansuc ...
Bacillus subtilis
J. Biochem.
85
287-293
1979
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488322
Pabst
The cell wall-associated levan ...
Actinomyces viscosus
Biochim. Biophys. Acta
566
274-282
1979
-
-
-
-
-
-
1
1
2
-
2
-
-
2
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
2
-
-
-
-
1
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488323
Figures
Resolution of the glycosyltran ...
Streptococcus mutans
Biochim. Biophys. Acta
577
142-146
1979
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488319
Tanaka
-
Levansucrase of Bacillus subti ...
Bacillus subtilis
Agric. Biol. Chem.
42
323-326
1978
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
4
-
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-
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-
1
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
4
-
1
-
2
-
1
-
-
-
-
-
-
-
488311
Chambert
Kinetic studies of levansucras ...
Bacillus subtilis
Eur. J. Biochem.
41
285-300
1974
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488315
Berthou
Letter: Crystallography of Bac ...
Bacillus subtilis, Bacillus subtilis BS5C4
J. Mol. Biol.
82
111-113
1974
-
-
-
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488308
Reese
Purification of levansucrase b ...
Aerobacter levanicum
Biochim. Biophys. Acta
113
79-83
1966
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
1
1
3
-
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