BRENDA - Enzyme Database show
show all sequences of 2.4.1.10

Two active forms of Zymomonas mobilis levansucrase: an ordered microfibril structure of the enzyme promotes levan polymerization

Goldman, D.; Lavid, N.; Schwartz, A.; Shoham, G.; Danino, D.; Shoham, Y.; J. Biol. Chem. 283, 32209-32217 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21(DE3) via the T7 RNA polymerase expression system with pET9d
Zymomonas mobilis
Engineering
Amino acid exchange
Commentary
Organism
H296R
no ability of the enzyme to form microfibrils and to synthesize levan at pH 6.0
Zymomonas mobilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
sucrose
substrate inhibition
Zymomonas mobilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
32 mM (sucrose), 25C, pH 5.0, total activity; at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity
Zymomonas mobilis
36
-
sucrose
25C, pH 5.0, transfructosylation activity
Zymomonas mobilis
40
-
sucrose
25C, pH 5.0, levan synthesis activity is monitored by following the increase in turbidity at 600 nm that occurrs due to the insoluble levan formation
Zymomonas mobilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
at pH 6.0 apparent MW greater than 1000000 Da
Zymomonas mobilis
94000
-
at pH 7.4, purification procedure includes two steps, MnCl2 precipitation and gel filtration, resulting in a 98% pure protein based on SDS-PAGE, calculated MW is 46700 Da, the enzyme appears to be a dimer in solution
Zymomonas mobilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zymomonas mobilis
Q60114
sacB gene, AAA27695; ATCC10988, expression in Escherichia coli BL21(DE3)
-
Purification (Commentary)
Commentary
Organism
The cell extract is centrifuged (14,000xg for 15 min) and the soluble supernatant is kept at 4C. Following the procedure of Vigants, the enzyme is selectively precipitated by adding 0.1 M MnCl2. Next, the solution is centrifuged (14,000xg for 15 min), the pellet is re-suspended in buffer B (50 mM phosphate buffer pH 6.0, 100 mM NaCl and 0.02% sodium azide), and then the soluble protein is passed via a Sephacryl S300 26/60 gel filtration column, running at 1 ml/min with either buffer A at pH 7.4 or with buffer B at pH 6.0.
Zymomonas mobilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
change in substrate specificity appears to be unique to Zymomonas mobilis levansucrase because the levansucrases from Bacillus subtilis, Rahnella aquatilis, and Lactobacillus reuteri are capable of synthesizing levan in their soluble form and are not reported to form fibril-like structures
687775
Zymomonas mobilis
?
-
-
-
-
sucrose + sucrose
dimer form
687775
Zymomonas mobilis
D-glucose + (2,6-beta-D-fructosyl)3
degree of polymerisation 3
-
-
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
pH lower than 7.0, fibril form
687775
Zymomonas mobilis
D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
structure depends on ionic strength and pHs, above 7.0, products are short fructo-saccharides (degree of polymerization 3)
Zymomonas mobilis
More
microfibrils, structure depends on ionic strength and pHs, below 6.0, well-ordered, precipate out of the solution, synthesis of levan (degree of polymerization greater than 20000). The fibril structure decreases the diffusion of the enzyme away from the bacteria cells, which results in the close association of the cell to the high molecular polymer, levan.
Zymomonas mobilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
379
-
sucrose
transfructosylation activity
Zymomonas mobilis
411
-
sucrose
levan synthesis activity
Zymomonas mobilis
461
-
sucrose
total activity
Zymomonas mobilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
Zymomonas mobilis levansucrase exists in two active forms, depending on the pH and ionic strength
Zymomonas mobilis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2000
-
sucrose
-
Zymomonas mobilis
2100
-
sucrose
-
Zymomonas mobilis
2800
-
sucrose
-
Zymomonas mobilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21(DE3) via the T7 RNA polymerase expression system with pET9d
Zymomonas mobilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H296R
no ability of the enzyme to form microfibrils and to synthesize levan at pH 6.0
Zymomonas mobilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
sucrose
substrate inhibition
Zymomonas mobilis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2000
-
sucrose
-
Zymomonas mobilis
2100
-
sucrose
-
Zymomonas mobilis
2800
-
sucrose
-
Zymomonas mobilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
32 mM (sucrose), 25C, pH 5.0, total activity; at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity
Zymomonas mobilis
36
-
sucrose
25C, pH 5.0, transfructosylation activity
Zymomonas mobilis
40
-
sucrose
25C, pH 5.0, levan synthesis activity is monitored by following the increase in turbidity at 600 nm that occurrs due to the insoluble levan formation
Zymomonas mobilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
at pH 6.0 apparent MW greater than 1000000 Da
Zymomonas mobilis
94000
-
at pH 7.4, purification procedure includes two steps, MnCl2 precipitation and gel filtration, resulting in a 98% pure protein based on SDS-PAGE, calculated MW is 46700 Da, the enzyme appears to be a dimer in solution
Zymomonas mobilis
Purification (Commentary) (protein specific)
Commentary
Organism
The cell extract is centrifuged (14,000xg for 15 min) and the soluble supernatant is kept at 4C. Following the procedure of Vigants, the enzyme is selectively precipitated by adding 0.1 M MnCl2. Next, the solution is centrifuged (14,000xg for 15 min), the pellet is re-suspended in buffer B (50 mM phosphate buffer pH 6.0, 100 mM NaCl and 0.02% sodium azide), and then the soluble protein is passed via a Sephacryl S300 26/60 gel filtration column, running at 1 ml/min with either buffer A at pH 7.4 or with buffer B at pH 6.0.
Zymomonas mobilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
change in substrate specificity appears to be unique to Zymomonas mobilis levansucrase because the levansucrases from Bacillus subtilis, Rahnella aquatilis, and Lactobacillus reuteri are capable of synthesizing levan in their soluble form and are not reported to form fibril-like structures
687775
Zymomonas mobilis
?
-
-
-
-
sucrose + sucrose
dimer form
687775
Zymomonas mobilis
D-glucose + (2,6-beta-D-fructosyl)3
degree of polymerisation 3
-
-
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
pH lower than 7.0, fibril form
687775
Zymomonas mobilis
D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
structure depends on ionic strength and pHs, above 7.0, products are short fructo-saccharides (degree of polymerization 3)
Zymomonas mobilis
More
microfibrils, structure depends on ionic strength and pHs, below 6.0, well-ordered, precipate out of the solution, synthesis of levan (degree of polymerization greater than 20000). The fibril structure decreases the diffusion of the enzyme away from the bacteria cells, which results in the close association of the cell to the high molecular polymer, levan.
Zymomonas mobilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
379
-
sucrose
transfructosylation activity
Zymomonas mobilis
411
-
sucrose
levan synthesis activity
Zymomonas mobilis
461
-
sucrose
total activity
Zymomonas mobilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
Zymomonas mobilis levansucrase exists in two active forms, depending on the pH and ionic strength
Zymomonas mobilis
Other publictions for EC 2.4.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735834
Ishida
Levansucrase from Leuconostoc ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides NTM048
Biotechnol. Lett.
38
681-687
2016
-
-
1
-
-
-
10
-
-
3
-
2
-
6
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
-
3
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735552
Li
Recent novel applications of l ...
Bacillus licheniformis, Bacillus subtilis, Microbacterium laevaniformans
Appl. Microbiol. Biotechnol.
99
6959-6969
2015
-
3
-
-
-
-
-
-
3
-
-
3
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
735800
Li
Purification and characterizat ...
Bacillus velezensis, Bacillus velezensis SK 21.002
Biotechnol. Appl. Biochem.
62
815-822
2015
-
-
-
-
-
-
7
2
2
2
1
2
-
4
-
-
1
-
-
-
1
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
2
2
1
2
-
-
-
1
-
-
1
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
735857
Mehmood
Expression of extra-cellular l ...
Pseudomonas savastanoi pv. glycinea, Pseudomonas savastanoi pv. glycinea PG4180
BMC Microbiol.
15
48
2015
-
-
1
-
-
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
735888
Porras-Dominguez
Bacillus subtilis 168 levansuc ...
Bacillus subtilis 168, Bacillus subtilis
Carbohydr. Polym.
132
338-344
2015
-
-
1
-
-
-
-
1
-
-
-
2
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735890
Li
Bacillus amyloliquefaciens lev ...
Bacillus amyloliquefaciens
Carbohydr. Polym.
133
203-212
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735894
Wu
Biosynthesis of lactosylfructo ...
Bacillus velezensis, Bacillus velezensis SK 21.002
Carbohydr. Res.
401
122-126
2015
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
736295
Li
Efficient biosynthesis of lact ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B?512
J. Agric. Food Chem.
63
9755-9763
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736648
Santos-Moriano
-
Levan versus fructooligosaccha ...
Zymomonas mobilis
J. Mol. Catal. B
119
18-25
2015
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
736704
Wuerges
The crystal structure of Erwin ...
Erwinia amylovora, Erwinia amylovora ATCC 49946
J. Struct. Biol.
191
290-298
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
736891
Visnapuu
Levansucrases of a Pseudomonas ...
Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis, Burkholderia cepacia, Dactylis glomerata, Erwinia amylovora, Gluconacetobacter diazotrophicus, Lactobacillus gasseri, Lactobacillus panis, Lactobacillus reuteri, Lactobacillus sanfranciscensis, Phleum pratense, Pseudomonas chlororaphis subsp. aurantiaca, Pseudomonas syringae, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000, Zymomonas mobilis
New Biotechnol.
32
597-605
2015
-
-
-
2
35
-
2
4
16
-
-
-
-
21
-
-
-
-
-
-
-
-
38
-
6
-
-
3
3
-
-
-
3
-
-
-
-
-
-
2
35
-
-
3
3
4
17
-
-
-
-
-
-
-
-
-
-
-
38
-
6
-
-
3
4
-
-
-
-
19
21
-
-
-
730222
Tian
-
Optimization of levansucrase/e ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens ATCC 23350
J. Mol. Catal. B
109
85-93
2014
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
736695
Rasmussen
Transcript profiling of fructa ...
Lolium perenne
J. Plant Physiol.
171
475-485
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736849
Mardo
High-throughput assay of levan ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Molecules
19
8434-8455
2014
-
1
1
-
31
-
-
-
1
-
-
2
-
6
-
-
-
-
-
-
2
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
31
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
737179
Lu
-
A recombinant levansucrase fro ...
Bacillus licheniformis, Bacillus licheniformis 8-37-0-1
Process Biochem.
49
1503-1510
2014
-
-
1
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
-
-
1
-
10
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
1
-
10
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
736130
Vigants
An influence of ethanol and te ...
Zymomonas mobilis 113S, Zymomonas mobilis
Folia Microbiol. (Praha)
58
75-80
2013
1
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
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1
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1
1
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736970
Verspreet
Fructan metabolism in developi ...
Triticum aestivum
Plant Cell Physiol.
54
2047-2057
2013
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1
1
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704960
Rairakhwada
Gene cloning, characterization ...
Bacillus amyloliquefaciens
J. Ind. Microbiol. Biotechnol.
37
195-204
2010
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1
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1
2
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1
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701567
El-Refai
-
Immobilization and properties ...
Bacillus circulans
Acta Pharm. Sci.
51
149-156
2009
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1
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5
1
1
1
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1
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1
1
3
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2
1
1
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701787
Menendez
Transcriptional regulation and ...
Gluconacetobacter diazotrophicus
Appl. Environ. Microbiol.
75
1782-1785
2009
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1
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1
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1
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701837
Chiang
Enhanced levan production usin ...
Zymomonas mobilis
Appl. Microbiol. Biotechnol.
82
445-451
2009
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1
1
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1
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1
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3
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1
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1
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1
1
-
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701838
Kang
Synthesis and characterization ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
Appl. Microbiol. Biotechnol.
83
1009-1016
2009
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1
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2
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702880
Ortiz-Soto
Evaluation of cross-linked agg ...
Bacillus subtilis
BMC Biotechnol.
9
68-75
2009
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3
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2
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2
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703493
Nam
-
Synthesis, structural analysis ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
Enzyme Microb. Technol.
45
247-252
2009
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1
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3
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2
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1
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-
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705086
Han
Production of lactosucrose fro ...
Zymomonas mobilis
J. Microbiol. Biotechnol.
19
1153-1160
2009
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1
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1
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2
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2
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1
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1
-
1
-
-
-
-
-
-
-
706683
Visnapuu
Fully automated chip-based neg ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Rapid Commun. Mass Spectrom.
23
1337-1346
2009
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1
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4
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8
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1
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1
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6
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1
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1
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1
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4
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1
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1
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6
-
1
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1
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-
-
-
-
706915
El-Refai
-
Enzymic synthesis of levan and ...
Bacillus circulans
World J. Microbiol. Biotechnol.
25
821-827
2009
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1
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2
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1
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1
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1
1
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1
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1
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1
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2
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1
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1
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3
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
684132
Li
Amino acid substitutions of Hi ...
Zymomonas mobilis
Acta Biochim. Pol.
55
201-206
2008
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1
-
10
-
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2
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1
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1
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10
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1
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684765
Waldherr
Genetic and functional charact ...
Lactobacillus panis
Arch. Microbiol.
190
497-505
2008
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1
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1
1
-
1
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1
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2
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1
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6
-
1
3
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1
1
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1
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1
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1
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1
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1
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-
6
-
1
3
-
-
1
1
-
-
-
-
-
-
-
-
685846
Meng
Donor substrate recognition in ...
Bacillus subtilis
BMC Struct. Biol.
8
16
2008
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1
1
3
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1
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1
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4
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2
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3
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1
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2
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687775
Goldman
Two active forms of Zymomonas ...
Zymomonas mobilis
J. Biol. Chem.
283
32209-32217
2008
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1
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1
3
1
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2
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1
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3
2
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3
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1
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3
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1
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1
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1
3
3
1
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2
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1
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3
2
-
-
-
3
-
1
-
-
-
-
-
-
-
-
689854
Ortiz-Soto
Selected mutations in Bacillus ...
Bacillus subtilis
Protein Eng. Des. Sel.
21
589-595
2008
2
-
1
1
13
-
-
10
-
-
-
-
-
3
-
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1
-
-
-
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-
4
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2
10
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2
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1
-
1
13
-
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10
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1
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4
-
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-
2
10
-
-
-
-
-
-
-
-
-
-
701566
Abdel-fattah
-
Biosynthesis of levansucrase b ...
Bacillus circulans
Acta Pharm. Sci.
50
203-212
2008
-
-
-
-
1
-
-
-
-
-
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1
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1
1
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1
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1
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1
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1
1
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1
-
-
-
1
-
-
-
-
-
-
-
-
-
702972
Esawy
-
Immobilisation of Bacillus sub ...
Bacillus subtilis, Bacillus subtilis NRC33a
Braz. J. Chem. Eng.
25
237-246
2008
-
-
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1
-
8
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1
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2
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1
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-
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1
1
1
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1
1
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1
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-
8
-
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1
-
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1
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-
-
-
-
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
671551
Biedendieck
Export, purification, and acti ...
Lactobacillus reuteri, Lactobacillus reuteri 21
Appl. Microbiol. Biotechnol.
74
1062-1073
2007
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1
-
-
-
-
-
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5
-
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1
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-
-
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6
-
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1
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1
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-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678072
Homann
Insights into polymer versus o ...
Bacillus megaterium, Bacillus megaterium DSM 319
Biochem. J.
407
189-198
2007
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-
1
-
12
1
-
8
1
-
1
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-
9
-
-
1
-
-
1
-
2
12
-
1
-
1
11
1
1
-
-
-
-
-
-
-
1
-
-
12
1
-
-
-
8
1
-
1
-
-
-
-
1
-
1
-
2
12
-
1
-
1
11
1
1
-
-
-
-
-
-
-
-
688315
Kang
Heterologous expression and op ...
Pseudomonas chlororaphis subsp. aurantiaca, Pseudomonas chlororaphis subsp. aurantiaca S-4380
J. Microbiol. Biotechnol.
17
1751-1757
2007
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1
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1
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5
-
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1
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2
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1
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1
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689722
Szwengiel
-
Levan synthesis during associa ...
Bacillus subtilis
Pol. J. Food Nutr. Sci.
57
433-440
2007
-
1
-
-
-
1
1
-
-
1
-
-
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1
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1
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1
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1
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1
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671449
Malten
A Bacillus megaterium plasmid ...
Lactobacillus reuteri 121, Lactobacillus reuteri
Appl. Environ. Microbiol.
72
1677-1679
2006
-
-
1
-
-
-
-
-
1
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-
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-
10
-
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1
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-
1
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1
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1
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1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672929
Seibel
Synthesis of sucrose analogues ...
Bacillus subtilis 168, Bacillus subtilis
Carbohydr. Res.
341
2335-2349
2006
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-
1
-
-
-
-
-
-
-
1
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5
-
-
-
-
-
-
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-
11
1
-
-
-
-
-
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-
-
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-
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-
1
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-
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1
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-
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-
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673743
Li
Thermo-responsive expression a ...
Pseudomonas syringae
FEMS Microbiol. Lett.
265
178-185
2006
-
-
-
-
-
-
-
-
1
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1
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3
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675816
van Hijum
Structure-function relationshi ...
Bacillus subtilis, Gluconacetobacter diazotrophicus, Lactobacillus sp., Streptococcus salivarius, Streptococcus sp., Zymomonas mobilis
Microbiol. Mol. Biol. Rev.
70
157-176
2006
-
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1
3
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6
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1
3
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675837
Ozimek
The levansucrase and inulosucr ...
Lactobacillus reuteri 121, Lactobacillus reuteri
Microbiology
152
1187-1196
2006
-
-
-
-
-
-
-
-
-
-
-
-
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10
-
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-
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-
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2
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675845
Laue
Contribution of alginate and l ...
Pseudomonas syringae
Microbiology
152
2909-2918
2006
-
-
-
-
-
-
-
-
-
-
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1
-
3
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-
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2
-
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1
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-
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-
1
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658750
Ozimek
Mutational analysis of the rol ...
Lactobacillus reuteri 121, Lactobacillus reuteri
FEBS Lett.
579
1124-1128
2005
-
-
1
-
2
-
-
-
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1
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-
-
13
-
-
1
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-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
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-
2
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1
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-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
671507
Tieking
Molecular and functional chara ...
Lactobacillus sanfranciscensis, Lactobacillus sanfranciscensis TMW 1.392
Appl. Microbiol. Biotechnol.
66
655-663
2005
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2005
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Cloning and expression of leva ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides B-512 FMC
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2005
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672734
Park
Lactosucrose production by var ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens IFO15535, Bacillus subtilis, Geobacillus stearothermophilus, Geobacillus stearothermophilus ATCC 12980, Paenibacillus polymyxa, Paenibacillus polymyxa KCCM35411, Pseudomonas syringae, Pseudomonas syringae IFO14086, Rahnella aquatilis, Rahnella aquatilis KCTC2858, Sterigmatomyces elviae, Sterigmatomyces elviae ATCC 18894
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Increasing the stability of sa ...
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2005
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Rahnella aquatilis, Rahnella aquatilis ATCC 15552
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2003
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488329
Ben Ammar
Characterization of a thermost ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TH4-2
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99
111-119
2002
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488343
Yanase
Identification of functionally ...
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2002
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488327
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Fructo-oligosaccharides produc ...
Gluconacetobacter diazotrophicus
Enzyme Microb. Technol.
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2001
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Roles of fructosyltransferase ...
Actinomyces naeslundii, Actinomyces naeslundii WVU45
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Jang
-
Comparison of characteristics ...
Zymomonas mobilis
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2001
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Synthesis of methyl beta-D-fru ...
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Refolding and purification of ...
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2000
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Leloup
Differential dependence of lev ...
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1999
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Hernandez
Levansucrase from Acetobacter ...
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1999
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Substitution of Asp-309 by Asn ...
Gluconacetobacter diazotrophicus
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1999
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Levansucrase of Rahnella aquat ...
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Euzenat
-
Production and purification of ...
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864
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1998
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Hochstrasser
Expression of a functional bar ...
Hordeum vulgare
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Sprenger
Fructan synthesis in transgeni ...
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Properties of levansucrase fro ...
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Purification and characterizat ...
Bacillus subtilis, Erwinia amylovora, Pseudomonas savastanoi pv. phaseolicola, Streptococcus mutans, Zymomonas mobilis
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Isolation and enzymic properti ...
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Ohtsuka
-
Characterization of levansucra ...
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Levansucrase from Zymomonas mo ...
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488321
Mntsl
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Comparison of levansucrase fro ...
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Pabst
The cell wall-associated levan ...
Actinomyces viscosus
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1979
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488323
Figures
Resolution of the glycosyltran ...
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1979
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Kinetic studies of levansucras ...
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Letter: Crystallography of Bac ...
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488308
Reese
Purification of levansucrase b ...
Aerobacter levanicum
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1966
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