Application | Comment | Organism |
---|---|---|
medicine | radiotherapy combining GnT-V down-regulation and cetuximab decelerates tumor growth | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene MGAT5, recombinant expression of enzyme Gnt-V in CNE-1 and CNE-2 cells using the lentiviral transfection method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GnT-V is used as a molecular target to further sensitize cetuximab-treated nasopharyngeal carcinoma (NPC) cells to radiation. Development of GnT-V-suppressed cell models, denoted CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224. Lentivirus-mediated shRNA inhibits GnT-V mRNA and protein expression in NPC cell lines. The expression levels of GnTV-V mRNA in these cell models were 36.3%, 33.9%, 42% and 35.5% of the levels found in the control groups, respectively. Similarly, the GnT-V protein levels in the CNE1-1564, CNE1-2224, CNE2-1564 and CNE2-2224 cell models are decreased by 60.13%, 59.89%, 43.67% and 46.18%, respectively. GnT-V expression and depletion of GnT-V in nasopharyngeal carcinoma cells, phenotypes, overview. Changes in the radiosensitivity of CNE-1 and CNE-2 cells are also related to PI3K/Akt signaling. The total Akt level does not vary among the different groups, but the phospho-Akt and phospho-PI3K levels present differences: the phospho-Akt and phospho-PI3K levels in CNE2-1564 and CNE2-2224 cells are lower than those in CNE-2-NC, and the levels detected in CNE2-1564/cetuximab and CNE2-2224/cetuximab are lower than those found in CNE2-1564 and CNE2-2224 cells. CNE2-NC/cetuximab cells present reduced levels of phospho-Akt and phospho-PI3K compared with CNE2-NC cells. The CNE-1 cells present similar results. In brief, the irradiation-induced alteration of beta-1,6 branches on the EGFR might influence PI3K/AKT signaling | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q09328 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CNE-1 cell | - |
Homo sapiens | - |
CNE-2 cell | - |
Homo sapiens | - |
HNE-1 cell | - |
Homo sapiens | - |
additional information | CNE-2 cells express 7.5fold and 2.30fold higher levels of GnT-V mRNA and protein than HNE-1 cells, and CNE-1 cells express 1.41fold and 1.36fold higher levels of GnT-V mRNA and protein than HNE-1 cells | Homo sapiens | - |
nasopharyngeal carcinoma cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
beta-1,6-N-acetylglucosaminyltransferase V | - |
Homo sapiens |
Mgat5 | - |
Homo sapiens |
N-acetylglucosaminyltransferase V | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of N-acetylglucosaminyltransferase V enhances the cetuximab-induced radiosensitivity of nasopharyngeal carcinoma (NPC) cells likely through EGFR N-glycan alterations. Cetuximab is an epidermal growth factor receptor (EGFR) inhibitor used as a radiosensitizer in the treatment of NPC. The half-maximal inhibitory concentration (IC50) of cetuximab in CNE-1 and CNE-2 cells is 0.717 and 1.244 mg/ml, respectively | Homo sapiens |
physiological function | N-acetylglucosaminyltransferase V (GnT-V) catalyzes the formation of the N-linked beta-1-6 branching of oligosaccharides adding GlcNAc to beta-1,6-linked branches | Homo sapiens |