Any feedback?
Literature summary for 2.4.1.178 extracted from
- Biosynthesis of cyanogenic glucosides: in vitro analysis of the glucosylation step (1984), Arch. Biochem. Biophys., 229, 177-186.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
UDP-glucose | 4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile | Triglochin maritima |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Triglochin maritima | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| High salt concentrations | 160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile | Triglochin maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
1 * 47000, SDS-PAGE | Triglochin maritima |
| 50000 | - |
gel filtration | Triglochin maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 4-hydroxymandelonitrile | Triglochin maritima | last step in biosynthesis of cyanogenic glucosides | UDP + taxiphyllin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triglochin maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes chromatography on Sephadex PD10 and DEAE-Sephadex | Triglochin maritima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Triglochin maritima | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.31 | - |
3,4-dihydroxymandelonitrile | Triglochin maritima |
| 0.94 | - |
4-hydroxymandelonitrile | Triglochin maritima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% glycerol, crude extract stable for several weeks | Triglochin maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 3,4-dihydroxymandelonitrile | - |
Triglochin maritima | UDP + 3,4-dihydroxymandelonitrile glucoside | - |
? | |
| UDP-glucose + 4-hydroxymandelonitrile | - |
Triglochin maritima | UDP + taxiphyllin | - |
? | |
| UDP-glucose + 4-hydroxymandelonitrile | last step in biosynthesis of cyanogenic glucosides | Triglochin maritima | UDP + taxiphyllin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Triglochin maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Triglochin maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Triglochin maritima |
| 8 | - |
assay at | Triglochin maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | about 50% of activity maximum at pH 6 and pH 9.5 | Triglochin maritima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
unstable below, crude enzyme extract | Triglochin maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
