General Stability | Organism |
---|---|
2-mercaptoethanol, 10 mM, improves stability in solution, 35% loss of activity after 24 h at 4°C | Avena sativa |
rather unstable when dissolved in buffer, 65% loss of activity after 24 h at 4°C | Avena sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Avena sativa | |
p-chloromercuribenzoate | - |
Avena sativa | |
Triton X-100 | - |
Avena sativa | |
UDP | - |
Avena sativa | |
UMP | - |
Avena sativa | |
Zn2+ | - |
Avena sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Avena sativa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | 62000 | gel filtration | Avena sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol | Avena sativa | participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B | UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | - |
oat | - |
Purification (Comment) | Organism |
---|---|
partial | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Avena sativa | - |
Storage Stability | Organism |
---|---|
-20°C, in the form of dry acetone powder, stable for several weeks | Avena sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose | i.e. tigogenin, at 12% of the activity with nuatigenin | Avena sativa | UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside | - |
? | |
(25R)-5alpha-spirostane-3beta,6alpha-diol + UDP-glucose | i.e. chlorogenin, at 30% of the activity with nuatigenin | Avena sativa | UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside | - |
? | |
(25R)-spirost-5-en-3beta-ol + UDP-glucose | i.e. diosgenin, at 17% of the activity with nuatigenin | Avena sativa | UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside | - |
? | |
20S,22S,25S-spirost-5-ene-3beta,25-diol + UDP-glucose | i.e. isonuatigenin, at 57% of the activity with nuatigenin | Avena sativa | UDP + 20S,22S,25S-spirost-5-ene-3beta,25-diol 3-O-beta-D-glucoside | - |
? | |
22S,25S-5alpha-tomatanin-3beta-ol + UDP-glucose | i.e. tomatidine, at 57% of the activity with nuatigenin | Avena sativa | UDP + 22S,25S-5alpha-tomatanin-3beta-ol 3-O-beta-D-glucoside | - |
? | |
22S,25S-solanid-5-enin-3beta-ol + UDP-glucose | i.e. solanidine, at 39% of the activity with nuatigenin | Avena sativa | UDP + gamma-chaconine | - |
? | |
androst-5-en-3beta-ol-17-one + UDP-glucose | i.e. androsterone, at 15% of the activity with nuatigenin | Avena sativa | UDP + androst-5-en-3beta-ol-17-one 3-O-beta-D-glucoside | - |
? | |
pregn-5-en-3beta-ol-20-one + UDP-glucose | i.e. pregnenolone, at 26% of the activity with nuatigenin | Avena sativa | UDP + pregn-5-en-3beta-ol-20-one 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol | i.e. nuatigenin, TDPglucose at 8%, ADPglucose at 11%, UDPgalactose at 40% the rate of UDP-glucose | Avena sativa | UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside | i.e. nuatigenin 3beta-D-monoglucoside | ? | |
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol | participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B | Avena sativa | UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Avena sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
unstable | Avena sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Avena sativa |