Literature summary for 2.4.1.195 extracted from

Reed, D.W.; Davin, L.; Jain, J.C.; Deluca, V.; Nelson, L.; Underhill, E.W.
Purification and properties of UDP-glucose: thiohydroximate glucosyltransferase from Brassica napus L. seedlings (1993), Arch. Biochem. Biophys., 305, 526-532.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	1.0 mM, relative activity 250%, 10 mM, relative activity 260%, in absence enzyme solutions will lose up to 35% activity in 1 week, activity is not recoverable by addition of 2-mercaptoethanol	Brassica napus
dithiothreitol	5.0 mM, relative activity 230%	Brassica napus

Application

Application	Comment	Organism
agriculture	glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways	Brassica napus
nutrition	glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways	Brassica napus

General Stability

General Stability	Organism
enzyme stability decreases in buffers at pH optimum 6.0	Brassica napus

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	-	Brassica napus
Cu2+	-	Brassica napus
Zn2+	-	Brassica napus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	phenylacetothiohydroximate	pH 6.0, 30°C	Brassica napus
0.46	-	UDPglucose	pH 6.0, 30°C	Brassica napus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	10 mM, relative activity 130%	Brassica napus
Mg2+	10 mM, relative activity 140%	Brassica napus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	1 * 46000, SDS-PAGE	Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDPglucose + phenylacetothiohydroximate	Brassica napus	second to last step in glucosinolate biosynthesis	UDP + desulfoglucotropeolin	-	?

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	-	cv Westar	-

Purification (Commentary)

Purification (Comment)	Organism
-	Brassica napus

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Brassica napus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.8	-	-	Brassica napus

Storage Stability

Storage Stability	Organism
0°C, storage at temperatures below with or without glycerol results in substantial loss of activity	Brassica napus
4°C, only a 5% loss of activity can be detected after 10 days	Brassica napus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the oxygen analog phenylacetohydroximate and the hydroxylated phenolic substances quercetin and caffeic acid are not glucosylated	Brassica napus	?	-	?
UDP-glucose + 2-(3-indolyl)acetothiohydroximate	-	Brassica napus	UDP + S-(alpha-D-glucopyranosyl)-2-(3-indoyl)acetothiohydroximate	-	?
UDP-glucose + 3-phenylpropanothiohydroximate	-	Brassica napus	UDP + S-(alpha-D-glucopyranosyl)-3-phenylpropanothiohydroximate	-	?
UDPglucose + phenylacetothiohydroximate	-	Brassica napus	UDP + desulfoglucotropeolin	-	?
UDPglucose + phenylacetothiohydroximate	second to last step in glucosinolate biosynthesis	Brassica napus	UDP + desulfoglucotropeolin	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 46000, SDS-PAGE	Brassica napus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	40	stable up to 30°C for at least 1 h, activity is lost quickly at 40°C or greater	Brassica napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Brassica napus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7.5	-	most stable in Tris buffer	Brassica napus

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Release 2023.1 (January 2023)