Cloned (Comment) | Organism |
---|---|
gene malQ, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Borreliella burgdorferi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Borreliella burgdorferi | A6YM39 | Lyme disease spirochaete, strain BB0166, gene malQ | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged MalQ from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Borreliella burgdorferi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MalQ is able to hydrolyse malto-oligosaccharides as well as to form their longer transglycosylation products and shows activity towards glucose, G1, and a series of malto-oligosaccharides, G2-G7, i.e. maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose | Borreliella burgdorferi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylomaltase | - |
Borreliella burgdorferi |
GH77 amylomaltase | - |
Borreliella burgdorferi |
MalQ | - |
Borreliella burgdorferi |
More | the enzyme belongs to the glycoside hydrolase family 77, together with GH13 and GH70, it forms the clan GH-H, known as the alpha-amylase family | Borreliella burgdorferi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Borreliella burgdorferi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Borreliella burgdorferi |