Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1-30 mM, 4fold-stimulation | Shigella boydii | |
dithiothreitol | 0.1-3 mM, about 5fold stimulation | Shigella boydii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Shigella boydii |
Protein Variants | Comment | Organism |
---|---|---|
D99A | highly active mutant enzyme | Shigella boydii |
E101A | mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+ | Shigella boydii |
E103A | highly active mutant enzyme | Shigella boydii |
K211A | greatly reduced activity, 60fold higher activity in the presence of Pb2+ than in the presence of Mn2+ | Shigella boydii |
R210A | active mutant enzyme | Shigella boydii |
R212A | active mutant enzyme | Shigella boydii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyphenylglyoxal | 0.2 mM, 50% inhibition | Shigella boydii | |
5,5'-dithio-bis-2-nitrobenzoic acid | 0.2 mM, 85% inhibition | Shigella boydii | |
diethyl dicarbonate | 0.2 mM 31% inhibition | Shigella boydii | |
Triton X-100 | as the concentration of Triton X-100 increases from 0 to 0.5% in the reaction mixture, the activity of the enzyme decreases but is maintained at about 60% of the original activity, up to 6% Triton X-100 | Shigella boydii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol | pH 7, 37°C | Shigella boydii | |
0.25 | - |
UDP-alpha-D-galactose | pH 7, 37°C | Shigella boydii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective | Shigella boydii | |
Mg2+ | 5 mM, highest stimulation of activityN in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective | Shigella boydii | |
Mn2+ | 5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective | Shigella boydii | |
Ni2+ | 5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective | Shigella boydii | |
Pb2+ | 5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective | Shigella boydii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Shigella boydii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Shigella boydii | the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Shigella boydii B14 | the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shigella boydii | - |
- |
- |
Shigella boydii B14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Shigella boydii |
Storage Stability | Organism |
---|---|
4°C, the crude enzyme preparation retains at least 60% of the initial activity after storage for up to 9 weeks | Shigella boydii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with: alpha- and beta-anomers of GlcNAc-benzyl, beta-anomers of GlcNBu-benzyl, GlcNAc-naphthyl, GlcNAc-isoquinolinyl, as well as GlcNAc-alpha-OCOCH2-COO(CH2)11-OPh and alpha- and beta-anomers of GlcNAc-(CH2)11-OPh | Shigella boydii | ? | - |
? | |
additional information | no activity with: alpha- and beta-anomers of GlcNAc-benzyl, beta-anomers of GlcNBu-benzyl, GlcNAc-naphthyl, GlcNAc-isoquinolinyl, as well as GlcNAc-alpha-OCOCH2-COO(CH2)11-OPh and alpha- and beta-anomers of GlcNAc-(CH2)11-OPh | Shigella boydii B14 | ? | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14 | Shigella boydii | UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14 | Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol | the synthetic acceptor substrate D-GlcNAc-alpha-PP-(CH2)11-O-Ph is an amphipathic molecule with similarity to the endogenous substrate D-GlcNAc-alpha-diphospho-ditrans,octacis-undecaprenol. The enzyme requires diphosphate in the acceptor substrate, Strict specificity for UDP-D-galactose as donor substrate | Shigella boydii | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol | the enzyme requires diphosphate in the acceptor substrate | Shigella boydii B14 | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol | - |
? | |
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol | synthetic acceptor. 11% of the activity with N-acetyl-D-glucosaminyl-alpha-diphospho-phenoxyundecanol | Shigella boydii | UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, calculated from sequence | Shigella boydii |
Synonyms | Comment | Organism |
---|---|---|
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase | - |
Shigella boydii |
UDP-Gal:GlcNAc-R 1,4-Gal-transferase | - |
Shigella boydii |
WfeD | - |
Shigella boydii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Shigella boydii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Shigella boydii |