Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon-Plus-RIL cells | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
UDP-alpha-D-glucose | at pH 7.5 and 30°C | Malus domestica | |
0.62 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is not affected by the addition of divalent cations (Mg2+ or Mn2+, 0-25 mM) or monovalent cations (Na+, 0-100 mM or K+, 0-10 mM) | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + phloretin | Malus domestica | - |
UDP + phlorizin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography and Superdex 200 gel filtration | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Malus domestica | - |
flower | highest expression | Malus domestica | - |
fruit | - |
Malus domestica | - |
leaf | - |
Malus domestica | - |
phloem | - |
Malus domestica | - |
root | - |
Malus domestica | - |
seed | - |
Malus domestica | - |
shoot | - |
Malus domestica | - |
xylem | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with caffeic acid, catechin, chlorogenic acid, 2-coumaric acid, 3-coumaric acid, 4-coumaric acid, cyanidin, 3-3,4 dihydroxyphenyl propionic acid, epicatechin, 3-hydroxybenzoic acid, naringenin, protocatechuic acid, quercetin and rutin | Malus domestica | ? | - |
? | |
UDP-alpha-D-galactose + phloretin | - |
Malus domestica | ? | - |
? | |
UDP-alpha-D-glucose + phloretin | - |
Malus domestica | UDP + phlorizin | - |
? | |
UDP-alpha-D-xylose + phloretin | - |
Malus domestica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53500, calculated from amino acid sequence | Malus domestica |
? | x * 59000, His6-tagged enzyme, SDS-PAGE | Malus domestica |
Synonyms | Comment | Organism |
---|---|---|
PGT1 | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Malus domestica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | - |
Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0972 | - |
phloretin | at pH 7.5 and 30°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | the enzyme shows significant activity from pH 7.0-8.0. Activity decreases to 20% at pH 6.0 and 9.0 and less than 5% at pH 5.0 and 10.0, respectively | Malus domestica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Malus domestica | calculated from amino acid sequence | - |
5.8 |