Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | the 155000 Da enzyme form is more sensitive to substrate inhibition than the 170000 Da precursor form | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
sucrose | 30°C, pH 5.2, 170000 Da precursor | Leuconostoc mesenteroides | |
34 | - |
sucrose | 30°C, pH 5.2, 155000 Da enzyme form | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides B-512F | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | change of molecular mass of the enzymeincubated for 60 days at room temperature. After storage, the 170000 Da dextransucrase precursor decreases in concentration as a consequence of proteolysis. Initially a mixture of the precursor and a 155000 Da form are found. After 60 days of incubation, the 155000 form is further digested to lower molecular mass species, particularly a 129000 Da form, which still retains dextransucrase activity | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
141 | - |
155000 Da enzyme form | Leuconostoc mesenteroides |
145 | - |
170000 Da precursor | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides B-512F | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Leuconostoc mesenteroides |