Cloned (Comment) | Organism |
---|---|
expression of the His4-tagged enzyme in Escherichia coli strain DH5alpha | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
sucrose | pH 6.7, 37°C, His4-tagged enzyme | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59300 | - |
x * 59300, recombinant His4-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | Leuconostoc mesenteroides NRRL B-742 | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides NRRL B-742 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His4-tagged enzyme from Escherichia coli | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
285 | - |
purified recombinant His4-tagged enzyme | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + CMP | - |
Leuconostoc mesenteroides | D-fructose + CMP 1-glucoside | - |
? | |
sucrose + CMP | - |
Leuconostoc mesenteroides NRRL B-742 | D-fructose + CMP 1-glucoside | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides NRRL B-742 | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59300, recombinant His4-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
742SPase | - |
Leuconostoc mesenteroides |
More | the enzyme belongs to family 13 of the glycosyl hydrolases | Leuconostoc mesenteroides |
SPase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant His4-tagged enzyme, stable up to | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
recombinant enzyme | Leuconostoc mesenteroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | recombinant His4-tagged enzyme, stable | Leuconostoc mesenteroides |