General Stability | Organism |
---|---|
strong susceptibility to photooxidation in presence of methylene blue. Maximal photoinactivation at pH 8.0, 85% loss of activity after only 1 min of irradiation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-ribose 1-phosphate | - |
Bos taurus | |
guanine | - |
Bos taurus | |
guanosine | - |
Bos taurus | |
hypoxanthine | - |
Bos taurus | |
PCMB | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Inosine | - |
Bos taurus | |
0.03 | - |
guanine | - |
Bos taurus | |
0.0435 | - |
guanosine | - |
Bos taurus | |
21.5 | - |
phosphate | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Bos taurus |
78000 | 80000 | gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | Theorell-Chance mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78.3 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine + phosphate | - |
Bos taurus | guanine + alpha-D-ribose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
rapid inactivation | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
guanine | with guanosine as variable substrate and 200 mM phosphate | Bos taurus | |
0.0145 | - |
guanine | with guanosine as variable substrate and 40 mM phosphate | Bos taurus | |
0.0313 | - |
guanosine | with guanine as variable substrate and 0.4 mM ribose 1-phosphate | Bos taurus | |
0.05 | - |
hypoxanthine | with guanosine as variable substrate and 200 mM phosphate | Bos taurus | |
0.065 | - |
guanine | with phosphate as variable substrate and 0.2 mM guanosine | Bos taurus | |
0.32 | - |
alpha-D-ribose 1-phosphate | with phosphate as variable substrate and 0.2 mM guanosine | Bos taurus | |
0.36 | - |
alpha-D-ribose 1-phosphate | with guanosine as variable substrate and 20 mM phosphate | Bos taurus |