Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudoalteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
phosphate | - |
Pseudoalteromonas sp. | |
0.32 | - |
Inosine | 37°C | Escherichia coli | |
0.34 | - |
phosphate | 37°C | Escherichia coli | |
0.67 | - |
Inosine | - |
Pseudoalteromonas sp. | |
0.83 | - |
Inosine | 50°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25018 | - |
x * 25018, calculated from sequence | Pseudoalteromonas sp. |
29000 | - |
x * 29000, SDS-PAGE | Pseudoalteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudoalteromonas sp. | A3F8V1 | Bsi590 | - |
Pseudoalteromonas sp. Bsi590 | A3F8V1 | Bsi590 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudoalteromonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-6-methylpurine nucleoside + phosphate | 13.2% of the activity with inosine | Pseudoalteromonas sp. | ? | - |
? | |
2'-deoxy-6-methylpurine nucleoside + phosphate | 13.2% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | ? | - |
? | |
2'-deoxyadenosine + phosphate | 13.4% of the activity with inosine | Pseudoalteromonas sp. | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyguanosine + phosphate | 3.7% of the activity with inosine | Pseudoalteromonas sp. | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyguanosine + phosphate | 3.7% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 15.4% of the activity with inosine | Pseudoalteromonas sp. | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 15.4% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
arabinofuranosyladenine + phosphate | 4.3% of the activity with inosine | Pseudoalteromonas sp. | ? | - |
? | |
guanosine + phosphate | 55.8% of the activity with inosine | Pseudoalteromonas sp. | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | 55.8% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Pseudoalteromonas sp. | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Pseudoalteromonas sp. Bsi590 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Pseudoalteromonas sp. |
? | x * 25018, calculated from sequence | Pseudoalteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
EcPNP | - |
Escherichia coli |
PiPNP | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Pseudoalteromonas sp. |
60 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | 15°C: about 45% of maximal activity, 50°C: about 45% of maximal activity | Pseudoalteromonas sp. |
25 | 70 | 25°C: about 40% of maximal activity, 70°C: about 60% of maximal activity | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
30 min, stable up to | Pseudoalteromonas sp. |
42 | - |
30 min, 26% loss of activity | Pseudoalteromonas sp. |
50 | - |
30 min, 15% loss of activity | Escherichia coli |
50 | - |
after 1 h of incubation at 50°C with 0.5 M KCl and 1 M KCl, the enzyme activities still retaines 81% and 38%, respectively. It is inactivated without KCl | Pseudoalteromonas sp. |
65 | - |
30 min, complete inactivation | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.93 | - |
phosphate | 37°C | Escherichia coli | |
44.93 | - |
Inosine | 37°C | Escherichia coli | |
104.9 | - |
phosphate | 50°C | Escherichia coli | |
104.9 | - |
Inosine | 50°C | Escherichia coli | |
113.6 | - |
phosphate | - |
Pseudoalteromonas sp. | |
113.6 | - |
Inosine | - |
Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Pseudoalteromonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
stable for 2.5 h | Pseudoalteromonas sp. |