Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R117E/K121E/D139R | the mutant shows greater stability compared with the wild type dimer and changes in its structures compared with the wild type dimer in a hexamer | Escherichia coli |
R117E/K121E/D139R/F120D | the mutant shows greater stability compared with the wild type dimer and changes in its structures compared with the wild type dimer in a hexamer | Escherichia coli |
R117E/K121E/D139R/F120D/I128S/F131G | the mutant shows greater stability compared with the wild type dimer and changes in its structures compared with the wild type dimer in a hexamer | Escherichia coli |
R117E/K121E/D139R/Y173S | the mutant shows greater stability compared with the wild type dimer and changes in its structures compared with the wild type dimer in a hexamer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABP8 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephacryl S-200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine + phosphate | - |
Escherichia coli | 7-methylguanine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | highest activity | Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PNP | - |
Escherichia coli |
purine nucleoside phosphorylase | - |
Escherichia coli |