Application | Comment | Organism |
---|---|---|
synthesis | potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine | Leishmania mexicana |
Cloned (Comment) | Organism |
---|---|
gene pdt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribosyl-base1 + base2 | Leishmania mexicana | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Leishmania mexicana U1103 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Leishmania mexicana MHOM | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Leishmania mexicana 2001 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Leishmania mexicana GT | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | E9AWJ0 | - |
- |
Leishmania mexicana 2001 | E9AWJ0 | - |
- |
Leishmania mexicana GT | E9AWJ0 | - |
- |
Leishmania mexicana MHOM | E9AWJ0 | - |
- |
Leishmania mexicana U1103 | E9AWJ0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxyadenosine + guanine | - |
Leishmania mexicana | 2',3'-dideoxyguanosine + adenine | - |
? | |
2',3'-dideoxyadenosine + guanine | - |
Leishmania mexicana U1103 | 2',3'-dideoxyguanosine + adenine | - |
? | |
2',3'-dideoxyadenosine + guanine | - |
Leishmania mexicana MHOM | 2',3'-dideoxyguanosine + adenine | - |
? | |
2',3'-dideoxyadenosine + guanine | - |
Leishmania mexicana 2001 | 2',3'-dideoxyguanosine + adenine | - |
? | |
2',3'-dideoxyadenosine + guanine | - |
Leishmania mexicana GT | 2',3'-dideoxyguanosine + adenine | - |
? | |
2',3'-dideoxyadenosine + hypoxanthine | high activity | Leishmania mexicana | 2',3'-dideoxyinosine + adenine | - |
? | |
2',3'-dideoxyadenosine + hypoxanthine | high activity | Leishmania mexicana U1103 | 2',3'-dideoxyinosine + adenine | - |
? | |
2',3'-dideoxyadenosine + hypoxanthine | high activity | Leishmania mexicana MHOM | 2',3'-dideoxyinosine + adenine | - |
? | |
2',3'-dideoxyadenosine + hypoxanthine | high activity | Leishmania mexicana 2001 | 2',3'-dideoxyinosine + adenine | - |
? | |
2',3'-dideoxyadenosine + hypoxanthine | high activity | Leishmania mexicana GT | 2',3'-dideoxyinosine + adenine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Leishmania mexicana | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Leishmania mexicana U1103 | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Leishmania mexicana MHOM | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Leishmania mexicana 2001 | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Leishmania mexicana GT | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + guanine | low activity | Leishmania mexicana | 2',3'-dideoxyguanosine + hypoxanthine | - |
? | |
2'-deoxyadenosine + guanine | - |
Leishmania mexicana | 2'-deoxyguanosine + adenine | - |
? | |
2'-deoxyguanosine + adenine | - |
Leishmania mexicana | 2'-deoxyadenosine + guanine | - |
? | |
2'-deoxyinosine + adenine | - |
Leishmania mexicana | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Leishmania mexicana U1103 | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Leishmania mexicana MHOM | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Leishmania mexicana 2001 | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Leishmania mexicana GT | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + guanine | - |
Leishmania mexicana | 2'-deoxyguanosine + hypoxanthine | - |
? | |
2'-fluoro-2'-deoxyadenosine + guanine | - |
Leishmania mexicana | 2'-fluoro-2'-deoxyguanosine + adenine | - |
? | |
2'-fluoro-2'-deoxyinosine + adenine | high activity | Leishmania mexicana | 2'-fluoro-2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-fluoro-2'-deoxyinosine + guanine | high activity | Leishmania mexicana | 2'-fluoro-2'-deoxyguanosine + hypoxanthine | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Leishmania mexicana | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Leishmania mexicana U1103 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Leishmania mexicana MHOM | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Leishmania mexicana 2001 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Leishmania mexicana GT | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
9-beta-D-arabinofuranosyl adenine + guanine | low activity | Leishmania mexicana | ? | - |
? | |
9-beta-D-arabinofuranosyl adenine + hypoxanthine | low activity | Leishmania mexicana | ? | - |
? | |
9-beta-D-arabinofuranosyl guanine + adenine | - |
Leishmania mexicana | ? | - |
? | |
9-beta-D-arabinofuranosyl guanine + hypoxanthine | low activity | Leishmania mexicana | ? | - |
? | |
9-beta-D-arabinofuranosyl hypoxanthine + adenine | - |
Leishmania mexicana | ? | - |
? | |
9-beta-D-arabinofuranosyl hypoxanthine + guanine | low activity | Leishmania mexicana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2'-deoxyribosyltransferase | - |
Leishmania mexicana |
LmPDT | - |
Leishmania mexicana |
PDT | - |
Leishmania mexicana |
type I NDT | - |
Leishmania mexicana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Leishmania mexicana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | over 50% of maximal activity within this range | Leishmania mexicana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme LmPDT remains stable during 36 h at different pH values | Leishmania mexicana |
95 | - |
purified recombinant enzyme LmPDT, 5 min, inactivation | Leishmania mexicana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Leishmania mexicana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | over 6% of maximal activity within this range | Leishmania mexicana |
General Information | Comment | Organism |
---|---|---|
physiological function | N-deoxyribosyltransferases catalyze the direct transfer of the 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor through a one-step reaction | Leishmania mexicana |