Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for in-flow synthesis of nucleoside analogues (2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives) of pharmaceutical interest in mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs), method, overview | Limosilactobacillus reuteri |
Cloned (Comment) | Organism |
---|---|
gene ndt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Limosilactobacillus reuteri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) for biosynthetic purposes, evaluation of performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest. Two biocatalytic routes based on nucleoside 2'-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and on uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) are investigated in the synthesis of 2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzes the synthesis of 5-fluoro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine in 65-59% conversion yield, while CpUP/AhPNP-IMER provides the best results for the preparation of arabinosyladenine with 60% conversion yield. Enzyme LrNDT is immobilized on an epoxy silica monolithic resin by an in situ procedure (30% for LrNDT immobilization rate) | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribosyl-base1 + base2 | Limosilactobacillus reuteri | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Limosilactobacillus reuteri SD2112 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Limosilactobacillus reuteri ATCC 55730 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | F8DQT0 | - |
- |
Limosilactobacillus reuteri ATCC 55730 | F8DQT0 | - |
- |
Limosilactobacillus reuteri SD2112 | F8DQT0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyuridine + adenine | immobilized recombinant enzyme, high activity | Limosilactobacillus reuteri | uracil + 2'-deoxyadenosine | - |
? | |
2'-deoxyuridine + adenine | immobilized recombinant enzyme, high activity | Limosilactobacillus reuteri SD2112 | uracil + 2'-deoxyadenosine | - |
? | |
2'-deoxyuridine + adenine | immobilized recombinant enzyme, high activity | Limosilactobacillus reuteri ATCC 55730 | uracil + 2'-deoxyadenosine | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Limosilactobacillus reuteri | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Limosilactobacillus reuteri SD2112 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Limosilactobacillus reuteri ATCC 55730 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
5-fluorouracil + 2'-deoxyadenosine | immobilized recombinant enzyme | Limosilactobacillus reuteri | 5-fluoro-2'-deoxyuridine + adenine | - |
? | |
5-fluorouracil + 2'-deoxyadenosine | immobilized recombinant enzyme | Limosilactobacillus reuteri SD2112 | 5-fluoro-2'-deoxyuridine + adenine | - |
? | |
5-fluorouracil + 2'-deoxyadenosine | immobilized recombinant enzyme | Limosilactobacillus reuteri ATCC 55730 | 5-fluoro-2'-deoxyuridine + adenine | - |
? | |
5-fluorouracil + 2'-deoxyuridine | immobilized recombinant enzyme | Limosilactobacillus reuteri | 5-fluoro-2'-deoxyuridine + uracil | - |
? | |
5-fluorouracil + 2'-deoxyuridine | immobilized recombinant enzyme | Limosilactobacillus reuteri SD2112 | 5-fluoro-2'-deoxyuridine + uracil | - |
? | |
5-fluorouracil + 2'-deoxyuridine | immobilized recombinant enzyme | Limosilactobacillus reuteri ATCC 55730 | 5-fluoro-2'-deoxyuridine + uracil | - |
? | |
5-iodouracil + 2'-deoxyadenosine | immobilized recombinant enzyme | Limosilactobacillus reuteri | 5-iodo-2'-deoxyuridine + adenine | - |
? | |
5-iodouracil + 2'-deoxyuridine | immobilized recombinant enzyme | Limosilactobacillus reuteri | 5-iodo-2'-deoxyuridine + uracil | - |
? | |
hypoxanthine + 2',3'-dideoxyuridine | - |
Limosilactobacillus reuteri | 2',3'-dideoxyinosine + uracil | - |
? | |
additional information | arabinosyluracil is a poor substrate for the immobilized recombinant enzyme | Limosilactobacillus reuteri | ? | - |
- |
|
additional information | arabinosyluracil is a poor substrate for the immobilized recombinant enzyme | Limosilactobacillus reuteri SD2112 | ? | - |
- |
|
additional information | arabinosyluracil is a poor substrate for the immobilized recombinant enzyme | Limosilactobacillus reuteri ATCC 55730 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
LrNDT | - |
Limosilactobacillus reuteri |
NDT | - |
Limosilactobacillus reuteri |
nucleoside 2'-deoxyribosyltransferase | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | assay at | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | assay at | Limosilactobacillus reuteri |